| 乳牙生理性吸收相关基因初探 |
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| 引用本文： | 张东林,吴明,唐小牛. 乳牙生理性吸收相关基因初探[J]. 皖南医学院学报, 2008, 27(2): 87-89 |
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| 作者姓名： | 张东林 吴明 唐小牛 |
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| 作者单位： | 1. 皖南医学院口腔系,安徽,芜湖,241002
2. 皖南医学院寄生虫学教研室,安徽,芜湖,241002 |
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| 基金项目： | 安徽省教育厅自然科学基金 |
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| 摘 要： | 目的:应用人类基因芯片技术对人乳牙生理性吸收的基因表达进行分析,筛选差异表达基因,以期研究牙根吸收的基因调控机制。方法:留取与乳牙根吸收部位相对应的软组织为实验组(A)及取适量切除的牙龈瓣为对照组(B),提取总RNA,逆转录为cDNA,用Cy3和Cy5荧光染料标记,与芯片杂交后扫描。结果:有明显差异表达的共有156条,其中表达上调的基因有91条;下调的基因有65条。结论:乳牙生理性吸收可能与多个基因相关。
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| 关 键 词： | 基因芯片 乳牙 生理性吸收 差异表达基因 |
| 文章编号： | 1002-0217(2008)02-0087-03 |
| 修稿时间： | 2007-12-04 |
Preliminary study on the relevant genes of physiological absorption of deciduous teeth |
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| ZHANG Dong-lin,WU Ming,TANG Xiao-niu. Preliminary study on the relevant genes of physiological absorption of deciduous teeth[J]. Acta Academiae Medicinae Wannan, 2008, 27(2): 87-89 |
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| Authors: | ZHANG Dong-lin WU Ming TANG Xiao-niu |
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| Abstract: | Objective:To screen the differentially expressed genes of physio-absorption of deciduous teeth by cDNA microarray technique for investigating the mechanism of the gene regulation of absorption during deciduous teeth.Methods:The total mRNAs were isolated from the tis- sues of corresponding root and gingiva and the RNAs were labeled with fluorescence Cy5 and Cy3 to prepare the cDNA probes which were then hybridized with the gene chip.Results:One hundred fifty six differen- tial expressed genes were shown in the tissues with up-regulation in 91 and down-regulation in 65.Conclusion:There are many genes correlat- ing to physiological absorption of deciduous teeth. |
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| Keywords: | cDNA microarray deciduous teeth physiological absorption differential expression gene |
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