DNA损伤修复基因多态性与308 nm准分子激光治疗白癜风疗效的相关性分析

目的]探讨DNA损伤修复基因多态性与308 nm准分子激光治疗白癜风疗效的相关性.方法]选择2015年7月至2016年7月确诊的白癜风患者66例,所有患者均接受308 nm准分子激光治疗,治疗前清晨空腹抽取患者外周血1 mL以提取基因组DNA,通过直接测序法对ERCC1 C118T、XPC Lys939Gln这2个SNP位点行基因分型,分析DNA损伤修复基因多态性与白癜风疗效的相关性.结果]66例患者治疗时间4个月及以下占39.39％,复色面积50％以上占34.85％,首次复色时间1个月及以下占75.76％.ERCC1 C118T rs11615与患者复色效果、首次复色时间及治疗时间均相关(P＜0.05),其中CC基因型复色面积50％以上、首次复色时间1个月及以下、治疗时间1个月及以下比例均明显大于CT+ TT基因型,差异有统计学意义(P＜0.05);而XPCLys939Gln该SNP位点与患者复色效果无关(P＞0.05).结论]ERCC1 C118T该SNP位点与接受308nm准分子激光治疗效果显著相关,相比CT+TT基因型,CC基因型复色效果更明显;XPC Lys939Gln该SNP位点对治疗效果影响不大.

Study on the Relationship between DNA Damage Repair Gene Polymorphism and 308nm Excimer Laser Treatment Effect for Vitiligo

Objective]To explore the relationship between DNA damage repair gene polymorphism and 308nm excimer laser treatment for vitiligo.Methods] A total of 66 patients diagnosed with vitiligo from July 2015 to July 2016 were selected as the research subjects.All patients received the 308nm excimer laser treatment.Peripheral blood (1 mL) was drawn on the fasting morning before treatment to extract genome DNA.Direct sequencing was used for genotype of two SNP sites (ERCC1 C118T and XPC Lys939Gln).The relationship between DNA damage repair gene polymorphism and laser treatment effect of vitiligo was analyzed.Results] Twenty-six out of sixty-six (39.39 ％) patients received less than or equal to 4 months treatment.Patients with more than 50％ of vitiligo area skin color restoring accounted for 34.85 ％.The first skin color restoring time for less than or equal one month accounted for 75.76 ％.ERCC1 C118T rs11615 was correlated with color restoring effect,first color restoring time,and duration of treatment (P＜0.05).The higher percentage of CC genotype of ERCC1 C118T SNP showed more efficacy in skin color recovery (restoring area ＞50％),shortening of first restoring color time (≤1 month),shorter time treatment (≤1 month) comparing to CT+TT genotype of this site (P＜0.05).XPC Lys939Gln SNP site had nothing to do with color restoring effect (P ＞0.05).Conclusion]ERCC1 C118T SNP site is significantly related to 308nm excimer laser treatment effect.Compared to the CT+TT genotype,the CC genotype of ERCC1 C118T SNP is more efficiency in color restoring effect.The XPC Lys939Gln SNP site has little effect on laser treatment effect.