骨肽注射液对维甲酸所致大鼠骨质疏松的治疗作用及其机制

[目的]探讨骨肽注射液对维甲酸所致大鼠骨质疏松的治疗作用及其机制.[方法]50只SD雄性大鼠随机分为正常对照组,模型对照组,骨肽注射液低、中、高剂量组,除正常组外,其余四组均连续2周每天注射70 mg/kg的维甲酸建模.骨肽注射液低、中、高剂量组大鼠灌胃给予100、200、400 μg/(kg·d)的骨肽注射液,正常对照组及模型对照组给予等量生理盐水,连续给药4周.观察并记录大鼠骨质量及骨密度,并采用全自动生化分析仪检测血钙、血磷含量及血清碱性磷酸酶(ALP)活性,试剂盒检测血清中抗酒石酸性磷酸酶(TRACP)活性及骨钙素水平.[结果]与正常对照组比较,模型对照组中骨质量,骨密度,血钙及血磷含量均显著降低(P<0.01),ALP及TRACP活性升高(P<0.01),骨钙素含量升高(P<0.01);与模型对照组比较,骨肽注射液低、中、高剂量组中骨质量、骨密度、血钙及血磷含量均明显提高(P<0.01),ALP、TRACP活性降低(P<0.01),骨钙素含量降低(P<0.01).[结论]骨肽注射液对维甲酸所致的大鼠骨质疏松具有一定的治疗作用,其机制与其调节骨代谢有关.

Mechanism and Effect Ossotide Injection on Osteoporosis induced by Retinoic Acid in Rats

[Objective]To explore the mechanism and effect of Ossotide injection on osteoporosis induced by retinoic acid in rats.[Methods]Fifty SD rats were randomly divided into normal control group,model control group,Ossotide injection low,medium and high groups.In addition to normal control group,and the other four groups were intraperitoneally injected with 70 mg/kg retinoic acid for 14 days.Ossotide injection low,medium and high groups were intragastrically administrated with 100,200,400 μg/(kg·d) Ossotide injection,the normal control group and the model control group were given equal amount of normal saline for 4 weeks.The rat bone mass,bone mineral density was observed and recorded.The level of calcium,phosphorus and alkaline phosphatase (ALP) in serum was detected by automatic biochemical analyzer method.The level of serum tartrate resistant acid phosphatase (TRACP) activity and osteocalcin content was detected by kit.[Results]Compared with normal group,the level of calcium and phosphorus in serum,bone mass,bone mineral density was decreased (P<0.01),the activity of ALP and TRACP was increased (P<0.01),the level of osteocalcin was increased in model group (P<0.01).Compared with model control group,the level of calcium and phosphorus,bone mass,bone mineral density in serum was increased (P<0.01),the activity of ALP and TRACP was decreased (P<0.01),the level of osteocalcin was decreased in Ossotide injection low,medium and high group (P<0.01).[Conclusion]Ossotide injection could treat retinoic acid induced osteoporosis in rats.