IL-13在急性肺损伤小鼠肺内的表达及其意义研究

[目的]观察急性肺损伤(ALI)小鼠肺内白介素-13(IL-13)表达及其在肺损伤炎症反应中的作用.[方法]采用气管注射脂多糖(LPS)复制ALI小鼠ALI动物模型,采用real-time PCR和ELISA法分别检测小鼠肺内IL-13 mRNA和支气管肺泡灌洗液(BALF)以及血清中IL-13含量.采用IL-13中和抗体预处理小鼠后,观察其对LPS诱导的小鼠ALI的炎症反应的影响,运用ELISA法检测小鼠BALF和血清中肿瘤坏死因子-α(TNF-α)和IL-1β的含量,HE染色观察肺组织形态学改变.[结果]与对照组相比,气管注射LPS诱导的ALI小鼠肺组织中IL-13 mRNA以及BALF和血清IL-13蛋白含量显著增加.与ALI组相比,IL-13中和抗体预处理组小鼠肺内TNF-α和IL-1β含量进一步增高,肺组织形态学损伤加重.[结论]ALI时小鼠肺内IL-13含量应激性增加,抑制IL13的受体活性,加重LPS诱导的小鼠ALI,提示IL-13是ALI内源性保护因子.

Expression and Role of IL-13 in Mice with Acute Lung Injury

[Objective]To investigate the expression of IL-13 (interleukin-13) and its effect on inflammatory reaction of acute lung injury (ALI) induced by LPS in mice.[Methods]LPS was intratracheally injected into mice to induce the ALI model.The real-time PCR and ELISA were used to detect IL-13 mRNA and protein expression in lung tissue,bronchoalveolar lavage fluid (BALF) and serum.The effects of IL-13 on ALI was investigated using IL-13 neutralizing antibody.The ELISA method was used to detect the content of TNF-α and IL-1β in BALF and serum.HE staining was used to observe pathological damage of lung tissue changes in mice.[Results]Compared with the control group,IL-13 mRNA expression in lung tissue and IL-13 protein expression in BALF and serum were increased markedly in ALI mice induced by LPS (P<0.05).Pretreatment of IL-13 neutralizing antibody increased the expression of TNF-α and IL-1β,and aggravated the changes in pathological damage of ALI mice (P<0.05).[Conclusion]IL-13 expression increases in ALI induced by LPS.While inhibition of IL-13 aggravates the damage in mouse with ALI induced by LPS,which indicates that IL-13 plays a protective role in ALI endogenously.