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| 实验性大鼠肝脏星状细胞的培养 | |
| 引用本文： | 李艳,侯毅鞠,张磊,韩超,赵龙,崔小键,张友良,于峰. 实验性大鼠肝脏星状细胞的培养[J]. 吉林医药学院学报, 2009, 30(1) |
| 作者姓名： | 李艳 侯毅鞠 张磊 韩超 赵龙 崔小键 张友良 于峰 |
| 作者单位： | 吉林医药学院,吉林,吉林,132013;吉林医药学院,吉林,吉林,132013;吉林医药学院,吉林,吉林,132013;吉林医药学院,吉林,吉林,132013;吉林医药学院,吉林,吉林,132013;吉林医药学院,吉林,吉林,132013;吉林医药学院,吉林,吉林,132013;吉林医药学院,吉林,吉林,132013 |
| 基金项目： | 吉林省教育厅科研规划项目 |
| 摘 要： | 目的完善大鼠肝星形细胞(HSC)的体外分离及培养方法,为肝纤维化的基础实验研究提供技术支持。方法选用Wistar大鼠经消化酶灌注肝脏后,用分离液分离HSC,通过观察其自发荧光及其显著特征性结构的脂肪染色、细胞免疫化学染色等方法鉴定。结果分离得到HSC,细胞得率为2×107/只,存活率为96%。结论通过本法成功分离及培养HSC,且方法经济、简便、稳定,有利于原代大鼠肝星状细胞的生物学研究。 |
| 关 键 词： | 肝脏星状细胞 细胞培养 鉴定 |
Culture and identification of hepatic stellate cells from experimental rat liver |
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| LI Yan,HOU Yi-ju,ZHANG Lei,HAN Chao,ZHAO Long,CUI Xiao-jian,ZHANG You-liang,YU Feng. Culture and identification of hepatic stellate cells from experimental rat liver[J]. Journal of Jilin Medical College, 2009, 30(1) | |
| Authors: | LI Yan HOU Yi-ju ZHANG Lei HAN Chao ZHAO Long CUI Xiao-jian ZHANG You-liang YU Feng |
| Affiliation: | Jilin Medical College;Jilin City;Jilin Province;132013;China |
| Abstract: | Objective To improve methods of extracorporeal isolation and culture of rat hepatic stellate cells.Methods HSC was islated with isolation liquid after the Wistar rats were given liver perfusion,methods of fat stains and cellular immunochemistry stains were identified through observation of its spontaneous fluorescence and significant features.Results The yield of HSC got by this method was about 2×107 per rat liver,purity and activity of cells were 96%.Conclusion This work providee a good and economic metho... |
| Keywords: | hepatic stellate cells cell culture identification |
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