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芦丁抑制家兔血小板激活因子诱导血小板活化作用的实验研究
引用本文:陈文梅,金鸣,吴伟,李金荣,臧宝霞,朴永哲. 芦丁抑制家兔血小板激活因子诱导血小板活化作用的实验研究[J]. 中国中西医结合杂志, 2002, 22(4): 283-285
作者姓名:陈文梅  金鸣  吴伟  李金荣  臧宝霞  朴永哲
作者单位:北京市心肺血管疾病研究所药理研究室,北京,100029
基金项目:国家自然科学基金课题(No.39670884),北京市跨世纪科技人才工程及北京市高科技实验室课题资助项目
摘    要:目的:观察芦丁对家兔血小板激活因子(PAF)诱导血小板聚集、5-羟色胺(5-HT)释放、血小板内游离钙离子浓度的影响及其机理。方法:以比浊法测定家兔洗涤血小板(WRR)聚集率,邻苯二甲醛(OPT)荧光分光光度法测定5-HT含量,以Fura-2荧光分光光度法测定血小板游离钙离子浓度的变化。结果:芦丁体外呈浓度依赖性抑制PAF(9.55×10^-9mol/L)诱发的WRP聚集、5-HT释放作用,IC50分别为0.73、1.13mmol/L;同时68.3、274、545μmol/L芦丁剂量依赖地抑制PAF(4.78×10^-9mol/L)引起的血小板内游离钙浓度增高。结论:芦丁可抑制PAF诱导的血小板聚集、5-HT释放及血小板内游离钙浓度增加。

关 键 词:血小板聚集  5-羟色胺  游离钙  芦丁  血小板激活因子  血小板活化  实验研究
修稿时间:2001-03-20

Experimental Study on Inhibitory Effect of Rutin against Platelet Activation Induced by Platelet Activating Factor in Rabbits
CHEN Wen mei,JIN Ming,WU Wei. Experimental Study on Inhibitory Effect of Rutin against Platelet Activation Induced by Platelet Activating Factor in Rabbits[J]. Chinese journal of integrated traditional and Western medicine, 2002, 22(4): 283-285
Authors:CHEN Wen mei  JIN Ming  WU Wei
Abstract:OBJECTIVE: To explore the inhibitory effect and mechanism of rutin against platelet activating factor (PAF) induced platelet aggregation, 5-HT release and intra-platelet free calcium concentration. METHODS: The rate of washed rabbit platelet (WRP) aggregation was measured by turbidimetry and O-phthaldialdehyde (OPT) fluoro-spectrophotometry (FSPM) was used to determine 5-HT content. The intraplatelet free calcium concentration was measured with Fura-2/AM FSPM assay. RESULTS: Rutin in vitro was concentration-dependently inhibiting PAF (9.55 x 10(-9) mol/L) induced WRP aggregation, the IC50 of 5-HT release was 0.73, 1.13 mmol/L respectively and the intraplatelet free calcium concentration elevation evoked by PAF (4.78 x 10(-10) mol/L) were inhibited by 68.3, 136, 274, 545 mumol/L of rutin dose-dependently. CONCLUSION: Rutin could inhibit PAF induced platelet aggregation, 5-HT release and the increase of intraplatelet free calcium.
Keywords:rutin   platelet aggregation   platelet activating factor   5 HT   free calcium
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