| 乙肝5项ELISA试剂盒CUTOFF值适用性的验证 |
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| 引用本文： | 吴子安,王栋,徐宁,易四维,李涛.乙肝5项ELISA试剂盒CUTOFF值适用性的验证[J].广东寄生虫学会年报,2012(10):1192-1194. |
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| 作者姓名： | 吴子安 王栋 徐宁 易四维 李涛 |
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| 作者单位： | 广东省中医院芳村分院检验科,广东广州510370 |
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| 摘 要： | 目的验证本实验室乙肝5项定性检测试剂盒的CUTOFF值是否适用于本实验室所属区域内的个体人群。方法测定120份标本HBsAg、HBeAg、HBsAb、HBeAb、HBcAb-IgG,计算其检测结果OD值的均值和标准差s。夹心法试剂盒计算（＋3s）结果与试剂盒提供的CUTOFF值进行比较,竞争法计算（-3s）结果与试剂盒提供的CUTOFF值进行比较。结果各夹心法检测项目HBsAg、HBsAb、HBeAg其（＋3s）均小于相应CUTOFF值,各竞争法检测项目HBeAb、HBcAb-IgG其（-3s）均大于其CUTOFF值。结论本实验室所使用的乙肝5项ELISA试剂盒所选择的CUTOFF值适用于本实验室所属区域内的99%正常人群。
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| 关 键 词： | 乙型肝炎病毒 酶联免疫吸附试验 CUTOFF值 |
Verification of the applicability of the CUTOFF value of the HBV ELISA kit |
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| WU Zi＇an,WANG Dong,XU Ning,YI Si-wei,LI Tao.Verification of the applicability of the CUTOFF value of the HBV ELISA kit[J].Journal of Tropical Medicine,2012(10):1192-1194. |
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| Authors: | WU Zi＇an WANG Dong XU Ning YI Si-wei LI Tao |
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| Institution: | (Clinical Laboratory of Fangcun Branch,Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangdong,Guangzhou 510370,China) |
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| Abstract: | Objective To verify the CUTOFF values of the HBV ELISA kit used in our laboratory. Methods To determine 120 samples with each HBV kit. Then to calculate OD value （＋3s） and then compared the CUTOFF value of each kit with the sandwich methods. To calculate OD value （-3s） and to compare with the CUTOFF value of each kit of competition methods. Results The value of （＋3s） of each sandwich method is less than the CUTOFF value of each kit. The value of （-3s） of each competitive method is also more than the CUTOFF value of each kit. Conclusion The CUTOFF value of the ELISA kit of HBV applies to 99% of the population coverd by our laboratory. |
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| Keywords: | HBV ELISA CUTOFF value |
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