γ -谷氨酰转移酶及同工酶对肝细胞癌变的监测作用

目的 探讨γ－谷氨酰转移酶（ＧＧＴ）异常表达在监测肝细胞癌变中的价值。方法 以２－乙酰氨基芴（２－ＦＡＡ）喂饲ＳＤ大鼠诱发肝癌，观察鼠肝细胞在癌变期间的病理组织学，酶组织化学，肝总ＲＮＡ水平，不同分子主同工酶的动态改变。结果 诱癌后随鼠肝组织学改变，肝ＧＧＴ过度表达，可溶性及膜结合性ＧＧＴ比活性均明显异常；肝总ＲＮＡ浓度与肝ＧＧＴ比活性或血清ＧＧＴ活笥均呈显著正相关；

Experimental study on value of carcino-embryonic gama-glutamyl transferase isoenzymes for monitoring carcinogensis of hepatocytes

Objective To explore the value of gama-glutamyl transferase (GGT) abnormal expression in monitoring carcinogensis of hepatocytes. Methods The kinetic alterations of rat (SD) liver pathology, enzymatic histochemistry, total RNA levels, different molecular form GGT and its isoenzymes were inveshgated on experimental hepatomas inducing with 0. 05% 2-fluoenylacetandde (2-FAA). Results The GGTs of livers were overexpressed ddring canceration, liver GGT specific achvihes (Ulg) including soluble and membrane-combined GGT achvihes of each group in experiment rats were significantly higher (q = 4.90, p < 0.01) than those of control groan. The total RNA concentalon was highly positively correlated with liver GGT specific achvities or serum GGT activities (r = 0.90, r = 0.79, P < 0.01). The mixed isoenzyme patterns of control rats' and embryo rats' GGT were found in sera and livers of experimental rats, the carcino-embryonic GGT bands can be detected at early stages of rat hepatocyte canceration. Conclusions The present data suggest that GGT is a sensitive enzyme for carcinogensis of hepatocytes, the analysis of carcino-embryonic GGT is a useful marker for early diagnosis of rat hepatoma.