非肥胖多囊卵巢综合征不孕患者瘦素mRNA和蛋白表达的对照研究

目的探讨非肥胖多囊卵巢综合征(PCOS)不孕患者黄素化颗粒细胞瘦素mRNA的表达和血清、卵泡液瘦素水平。方法对超促排卵条件下16例非肥胖PCOS不孕患者和24名正常对照进行研究。采用逆转录聚合酶链反应(RTPCR)半定量测定卵巢黄素化颗粒细胞瘦素mRNA的表达,用酶联免疫吸附法(ELISA)测定血清和卵泡液中瘦素蛋白水平的表达。结果(1)两组对象的卵巢黄素化颗粒细胞上均有瘦素mRNA的表达,但表达量差异无统计学意义(P>005)。(2)两组对象血清瘦素、卵泡液瘦素水平比较,差异均无统计学意义(均P>005)。(3)PCOS组患者血清瘦素与卵泡液瘦素水平比较,差异无统计学意义(P>005);对照组血清瘦素与卵泡液瘦素水平比较,差异也无统计学意义(P>005)。(4)两组对象中卵泡液瘦素水平均与血清瘦素水平显著正相关(PCOS组rs=0838,P=0000;对照组rs=0837,P=0000),血清瘦素水平均与体重指数显著正相关(PCOS组rs=0665,P=0005;对照组rs=0712,P=0000);(5)两组对象中卵巢黄素化颗粒细胞瘦素mRNA表达与卵泡液瘦素水平均无相关性(均P>005)。结论非肥胖PCOS患者卵巢黄素化颗粒细胞有瘦素mRNA的表达,其表达量无异常;非肥胖PCOS患者血清、卵泡液瘦素水平与正常妇女类似,血清瘦素主要来源于脂肪组织,卵泡液瘦素水平主要取决于循环瘦素水平。

Expression of leptin mRNA in luteinized granulosa cells and leptin levels in serum and follicular fluid of non-obese infertile patients with polycystic ovary syndrome

OBJECTIVE: To investigate the expression of leptin mRNA in luteinized granulose cells and the leptin level in serum and follicular fluid of non-obese patients with polycystic ovary syndrome (PCOS). METHODS: Granulosa cells were collected from the puncture fluid of follicle of 16 obese infertile patients with PCOS to undergo external fertilization. Fasting venous blood was collected while collecting the ova. RT-PCR was used to detect the expression of leptin mRNA in the granulosa cells. ELISA was used to detect the leptin level in the serum and follicular fluid. Twenty normal women undergoing external fertilization because of male infertility of their husbands were used as controls. RESULTS: The expression of leptin mRNA (A value) was 1.5 +/- 0.4 in the obese infertile patients with PCOS and was 1.8 +/- 0.6 (P > 0.05) in the controls. Both the leptin levels of serum and follicular fluid were not different between the obese infertile patients with PCOS and controls (both P > 0.05). The leptin level in follicular fluid was significantly correlated with both the serum leptin level in the obese infertile patients with PCOS (r(s) = 0.838, P = 0.000) and the control group (r(s) = 0.712, P = 0.000). The expression level of leptin mRNA in the luteinized was not correlated with the leptin level of follicular fluid in both the obese infertile patients with PCOS and the control group (both P > 0.05). The fertility rate was not different between the obese infertile patients with PCOS (7/16) and the controls (9/24) (P = 0.693). CONCLUSION: The expression of leptin mRNA is normal in the follicular fluid of the obese infertile patients with PCOS. The leptin levels in serum and follicular fluid of the obese infertile patients with PCOS are similar to those of normal women. The leptin level in follicular fluid depends mainly on the circulating leptin level.