曲美他嗪对慢性心力衰竭大鼠心肌能量代谢及超微结构的影响

目的研究曲美他嗪对异丙肾上腺素诱导的慢性心力衰竭大鼠心肌能量代谢和超微结构的影响。方法72只雄性SD大鼠随机分为对照组(14只)和模型组(58只),模型制备采用皮下多点注射异丙肾上腺素法,4周后模型组大鼠死亡12只,再将存活的46只模型组大鼠随机分为未治疗组(M组,16只)、曲美他嗪治疗组(T组,15只)和培哚普利治疗组(P组,15只)。治疗5周后行二维心脏超声检查和病理形态学观察,并测定心肌组织能量代谢相关指标。结果与M组比较,T组大鼠LVEF、左心室短轴缩短率均提高;光镜和电镜下观察T组和P组心肌损伤程度较M组明显减轻。T组与P组大鼠心肌二磷酸腺苷(ADP)、一磷酸腺苷、ATP/ADP和总腺苷含量与对照组比较差异无统计学意义(P>0.05)。T组大鼠心肌肌浆网Ca2+-ATPase活性与对照组和P组比较差异无统计学意义。结论曲美他嗪能够改善心力衰竭大鼠心肌能量代谢、病理和超微结构,并且有改善大鼠心功能的趋势。

Effects of trimetazidine on myocardial energy metabolism and ultrastructure in chronic heart failure rats

Objective To investigate the effects of trimetazidine,compared with perindopril,on myocardial energy metabolism and ultrastructure in chronic heart failure(CHF) rats.Methods Seventy-two male SD rats were randomly divided into control group and model group.Rats in model group were injected subcutaneously with isoproterenol.Four weeks later,the rats in model group were randomly divided into three groups:untreated group(group M),trimetazidine treated group(group T) and perindopril treated group(group P).After treatment for five weeks,echocardiography and myocardial pathological examination were performed.The energy metabolism indexes of myocardium were determined.Results Compared with the rats in group M,EF and FS of the rats in group T increased by 6.5% and 11.8% respectively.The level of ADP,AMP,ATP/ADP and TAN(total adenosine) had no significant differences among groups T,P and control.Group T showed a significant increase in myocardial sarcoplasmic reticulum Ca2 -ATPase activity as compared with group M,but there were no significant differences among groups T,P and control.The microscopic and electron microscopic examination showed that the myocardial injury in groups T and P was ameliorated as compared with that in group M.Conclusion Trimetazidine can improve myocardial energy metabolism,pathological change and ultrastructure and enhance the cardiac function in HF rats.