芦丁对大鼠缺血再灌注心肌组织iNOS和eNOSmRNA表达的影响

目的：研究芦丁对大鼠心肌缺血再灌注损伤的保护作用及其机制。方法通过左冠状动脉结扎建立SD大鼠心肌缺血再灌注模型，将大鼠随机分为对照组、模型组、芦丁组、L-NAME（eNOS 抑制剂）组，ELISA 法检测血清一氧化氮（NO）水平，免疫抑制法检测血清肌酸激酶同工酶（CK-MB）浓度，实时荧光定量 PCR分析各组心肌组织iNOS 和eNOS mRNA 表达的变化。结果与模型组比较，芦丁组血清 NO 水平增高（P＜0.01），CK-MB 浓度下降（P＜0.01），eNOS mRNA 表达升高（P＜0.01），iNOS mRNA 表达下降（P＜0.01）。结论芦丁缺血预处理对心肌起到了保护作用，其机制可能与上调心肌组织eNOS mRNA 、下调iNOS mRNA 表达有关。

Effect of Rutin on the Expression of eNOS and iNOS mRNA in Myocardial Tissues of Rats with Ischemia Reperfusion Injury

Objective To investigate the protective effect of Rutin on myocardial tissue of rats with ischemia-reperfusion injury.Methods Rat model of ischemia-reperfusion injury was set up by ligating the left anterior de-scending batch of the coronary artery.SD rats were randomly divided into control group ,model group ,Rutin group and L-NAME （eNOS inhibitor） group.Serum level of NO was determined by ELISA and CK-MB concentration was examined by immun osuppression assay.The expression of iNOS and eNOS mRNA in myocardial tissues was evaluated by real-time quantitative PCR analysis.Results Compared with model group ,serum level of NO was in-creased while CK-MB concentration was significantly decreased in rats of Rutin group （P 〈0.01）.The expression of eNOS mRNA was up-regulated while the expression of iNOS mRNA was down-regulated in myocardial tissues of rats in Rutin group （P〈0.01）.Conclusion Ischemic preconditioning with Rutin is effective in the treatment of ischemia-reperfusion injury.The mechanism might be associated with regulating the expressions of eNOS and iNOS mRNA in myocardial tissues.