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| RNAi抑制胰腺癌细胞survivin表达的实验研究 | |
| 引用本文： | 何满西,田伯乐,胡伟明,张肇达. RNAi抑制胰腺癌细胞survivin表达的实验研究[J]. 西部医学, 2009, 21(9): 1460-1463 |
| 作者姓名： | 何满西 田伯乐 胡伟明 张肇达 |
| 作者单位： | 1. 雅安市人民医院普外科,四川,雅安,625000 2. 四川大学华西医院普外科,四川,成都,610041 |
| 摘 要： | 目的研究用RNAi表达载体对胰腺癌细胞PANC-1中凋亡抑制蛋白survivin表达的抑制作用。方法用免疫荧光技术和RT-PCR检测胰腺癌细胞PANC-1中凋亡抑制蛋白survivin的表达，并把survivin基因克隆到T载体进行测序，构建针对survivin基因的干扰载体，用脂质体转染胰腺癌细胞PANC一1，RT-PCR初步分析干扰载体对survivin表达地抑制情况。结果survivin在胰腺癌细胞PANC-1中高表达，其基因序列与genebank中公布的一致，干扰载体si-svv-2可以有效的抑制survivin的表达，抑制率达71％。结论用RNAi表达载体可以有效抑制胰腺癌细胞PANC-1中凋亡抑制蛋白survivin的表达。 |
| 关 键 词： | RNAi 胰腺癌 survivin |
The study of inhibitting the expression of survivin in pancreatic cancer cells with RNAi |
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| MAN Xi-he,BO Le-tian,WEI Ming-hu,ZHAO Da-zhang. The study of inhibitting the expression of survivin in pancreatic cancer cells with RNAi[J]. , 2009, 21(9): 1460-1463 | |
| Authors: | MAN Xi-he BO Le-tian WEI Ming-hu ZHAO Da-zhang |
| Affiliation: | MAN Xi-he, ZHAO Da-zhang, BO Le-tian, et al (1. Department of General Sergery , People＇s Hospital of Ya ＇an , Ya ＇an 625000 ; Department of General Sergery ,West China Hospital ,Sichuan University, Chengdu 610041) |
| Abstract: | Objective To investigate the inhibitory effects of RNAi expression vector on the expression of survivin in pancreatic cancer cell PANC-1. Method The expression of survivin was examined with RT-PCR and immunofluorescence and the survivin gene was cloned into the T-vector and sequenced. At the same time,the RNAi expression vectors aimed survivin were constructed and transfected into PANC-1 cells with liposomes. The expression of survivin mRNA was detected with RT-PCR. Results There was a high degree expression of survivin in PANC-1 cells. The sequence was according with the survivin gene in GENE BANK. The expression of survivin was inhibited about 71% by si-svv-2 RNAi expression vectors. Conclusion The RNAi expression vector can effectively inhibit the expression of survivin in PANC-1 cells. |
| Keywords: | RNAi survivin |
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