核因子-κB反义RNA对自发性高血压大鼠血管平滑肌细胞增殖的作用

目的：探讨核因子（NF）-κB反义RNA重组腺病毒预处理对凝血酶诱导培养的自发性高血压大鼠（SHR）血管平滑肌细胞（VSMCs）增殖的影响。方法：分别用表达NF—κB反义RNA的50多重感染（MOI）重组腺病毒和100μmol／L吡咯烷二硫代氨基甲酸（PDTC，NF—κB特异性阻断剂）预处理VSMCs 48h和1h．采用WST-1试剂和氚胸腺嘧啶核苷（^3H—TdR）掺人率测定0．5U／ml凝血酶诱导的VSMCs细胞增殖率。结果：与PDTC一样。表达NF—κB反义RNA的重组腺病毒预处理明显抑制凝血酶诱导VSMCs的增殖（P〈0．01）．抑制率〉30％。结论：NF—κB反义RNA与NF—κB特异性阻断剂对VSMC的增殖具有相同的抑制作用，其抑制率超过30％。

Effect of antisense RNA of NF-κB on proliferation of vascular smooth muscle cells in spontaneously hypertensive rats

Objective： To investigate the effect of antisense RNA of NF-κB on proliferation of vascular smooth muscle cells （VSMCs） in spontaneously hypertensive rats （SHR） promoted by thrombin. Methods：After pretreated with the 50MOI recombinant adenovirus expressing antisense RNA of NF-κB for 48h and 100μmol/L PDTC （NF-κB special inhibitor） for lh, VSMCs proliferation induced by 0. 5U/ml thrombin was investigated. The proliferation rate of VSMCs was determined with both WST-- 1 metabolic activity and ^3 H--TdR incorporation efficiency. Results：The pretreatment of recombinant adenovirus expressing NF-κ B antisense RNA obviously inhibit VSMCs proliferation induced by thrombin （P〈0. 01） at the rate of 〉30%, which was similar to the inhibition of VSMCs proliferation by NF-κ B special inhibitor PDTC with no statistical difference between two group（P〉0.05）. Conclusion： The recombinant adenovirus expressing NF-κB antisense RNA may effectively inhibit VSMCs proliferation induced by thrombin,is similar to the NF-κB special inhibitor,may be applied to successive basic research and clinical interference treatment.