HPLC法测定土鳖虫中尿嘧啶和尿囊素的含量

目的建立土鳖虫药材中尿嘧啶和尿囊素的含量测定方法。方法采用高效液相色谱法,色谱柱为AlltimaNH2（150 mm×4.6 mm,5μm）;流动相为乙腈-水（90：10）;流速为1.0 ml/min,检测波长205 nm;柱温35℃。结果尿囊素和尿嘧啶的线性范围分别是：0.404~3.23μg（r=0.999 5,n=5）,0.820~6.56μg（r=0.999 5,n=5）。平均回收率分别为98.8%、99.0%,RSD分别为0.96%、0.79%（n=6）。结论该方法简便、准确,可作为土鳖虫药材的质量控制方法。

Determination of auantion and uracil in Eupolyphaga seu Steleophaga by HPLC

Objective To establish a method for determination of uracil and auantion in Eupolyphaga seu Steleophaga by HPLC.Methods The HPLC separation was performed on Alltima NH2（150 mm×4.6 mm,5 μm）column with a mixture of acetonitrile-water（9010）as the mobile phase,at a flow rate of 1.0 ml/min.Detection wavelength was at 205 nm,and column temperature was at 35 ℃.Results The linear response of Auantion and Uracil range from 0.404～3.23 μg（r=0.999 5,n=5）and 0.820～6.56 μg（r=0.999 5,n=5）;the average recovery was 98.8% with RSD 0.96% and 99.0% with RSD 0.79% respectively（n=6）.Conclusion The method is simple,accurate and suitable for the determination of auantion and uracil in Eupolyphaga seu Steleophaga.