钝顶螺旋藻酸性多糖的分离纯化和结构分析

本研究从钝顶螺旋藻中分离纯化了酸性多糖,并进行了结构分析.多糖经DEAE-纤维素柱和Sephadex-200凝胶柱纯化得到螺旋藻酸性多糖纯品,HPLC检测纯度达96.7%,分子量均一,约1.62×10~5.TLC和GC分析表明该多糖主要由D-鼠李糖、D-木糖、D-葡萄糖和D-甘露糖醛酸构成,摩尔比为65.5:19.5:9:6.经IR、~(13)C NMR和甲基化分析,推测该多糖可能以1,3-连接的鼠李糖为主链.其中木糖为β构型,鼠李糖、葡萄糖和甘露糖醛酸均为α构型,所有糖基均呈吡喃型.

Isolation, Purification and Structural Analysis of Acidic Polysaccharide from Spirulina Platensis

The acidic polysaccharide was isolated and purified from Spirulina Platensis.The pure acidic polysaccharide of Spirulina Platensis(APSP)was obtained after chromatography on DEAE-cellulose and Sephadex-200 columns.Its purity was 96.7%determined by HPLC,and the molecular weight was homogeneous.The mean MW was about 1.62×10~5.The components of APSP were identified by GC and TLC as:D-rhamnose,D-xylose,D-glucose and D-mannuronic acid in a molar ratio of about 65.5:19.5:9:6.The results of IR,~(13)C NMR and methylation analysis showed that APSP probably had a main chain of 1,3-linked rhamnose residues,and the xylose had β-configuration while the rhamnose,glucose,and mannuronic acid had α-configuration.The configuration of all residues was pyranose form.