微波法提取沙棘总黄酮的工艺研究

目的研究微波法提取沙棘总黄酮的最佳提取条件。方法首先用石油醚对沙棘进行脱脂处理,然后在单因素试验基础上,以乙醇浓度、微波功率、提取时间、料液比为因素,选取不同水平,以沙棘总黄酮提取率为评价指标,通过正交试验筛选出最佳提取工艺。结果实验选取的四个因素中,乙醇浓度和料液比对沙棘总黄酮提取率影响显著;微波法提取沙棘总黄酮的最佳提取工艺条件为乙醇浓度75%,微波功率300 W,提取时间2 min,料液为1∶20,沙棘总黄酮提取率最高可达3.9%。结论微波法提取沙棘总黄酮有时间短、提取效率高、对沙棘活性成分破坏较少等优点,可以为沙棘资源的综合开发提供参考。

Technical Study of the Microwave Extraction of the Total Flavonoids from Hippophae Rhamnoides

Objective To study the optimal extraction condition of the flavones from Hippophae Rhamnoide with microwave method.Methods The petroleum ether was used for the derosination of Hippophae Rhamnoides at first.Afterward,on the basis of the single-factor test,by taking ethanol concentration,microwave power,extraction time and feed liquid ratio as the factors,at different levels,taking the extraction rate of the total flavonoids from Hippophae Rhamnoides as the assessment indicator,the orthogonal test was applied to optimize the extraction techniques.Results Of the four factors optioned in the trial,the ethanol concentration,and feed liquid ratio presented the significant impacts on the extraction rate of the total flavonoids from Hippophae Rhamnoides.The impacts of microwave power and extraction time on the trial results were not significant.The optimal extraction technical conditions of the total flavonoids from Hippophae Rhamnoides were 75% of ethanol concentration,300 w in microwave power,2 min in the extraction time and 1∶ 20 in feed liquid ratio.The extraction rate of the total flavonoids from Hippophae Rhamnoides could be up to 3.9%.Conclusion The microwave extraction method is advantageous as short extraction time,high extraction efficiency and less destruction of the active ingredients of Chinese medicine in the extraction of the total flavonoids from Hippophae Rhamnoides.