中药颤复宁血清对6-羟基多巴诱导的PC12 细胞凋亡的抑制作用

目的：探讨中药颤复宁血清对6-羟基多巴（6-OHDA）诱导的大鼠嗜铬细胞瘤细胞（PC12）凋亡的抑制作用。方法：用血清药理学方法制备中药血清．加到经6-OHDA处理的PCI2细胞培养液中，用四甲基偶氮唑蓝（MTT）法检测细胞存活率．并用免疫组织化学染色方法和图像分析检测酪氨酸羟化酶（TH）含量及半胱氨酸蛋白酶3（Caspase-3）阳性凋亡细胞数。结果：颤复宁中药血清能有效地提高PC12细胞的存活率．对6-OHDA诱孚的凋亡有明显抑制作用。与6-OHDA损伤组比较，颤复宁血清组TH阳性细胞平均吸光度增高（P〈0．01），Caspase-3阳性细胞数明显降低（P〈0．01）。结论：中药颤复宁血清对PC12细胞具有直接的神经营养作用．能提高细胞存活率，且对6-OHDA诱导的细胞凋亡具有抑制作用。

Inhibitory effect of chanfuning serum on the apoptosis of PC12 cells induced by 6-hydroxydopamine

Objective: To study the inhibitory effect of chanfuning serum on the apoptosis of PC12 cells induced by 6-hydroxydopamine (6-OHDA). Methods: Serum pharmacological method was used to make chanfuning serum. 6-OHDA and chanfuning serum were added into the medium of PC12 cells separately. MTT method was used to assay the cell's viability, and tyrosine hydroxylase (TH) and caspase-3 immunocytochemistry staining were used to evaluate the content of TH and count the number of apoptosis cells. Results: PC12 cell's viability and calibrated optic density of TH positive cells enhanced (P<0.01), and the number of caspase-3 positive cells decreased(P<0.01) significantly in chanfuning serum treated group compared with 6-OHDA treated group. Conclusion: Chanfuning serum may have direct nutritious effect, protect PC12 cells from injury induced by 6-OHDA, and produce anti-apoptosis effect to some extent.