胶质细胞源性神经营养因子对大鼠脊髓损伤后运动功能的影响

目的:研究胶质细胞源性神经营养因子（GDNF）对大鼠脊髓损伤后后肢运动功能恢复的影响。方法:采用改良Nystr(?)m法后路压迫大鼠胸段脊髓造成损伤模型,经蛛网膜下腔置管局部连续给予NGF （10μg/d）或GDNF（10μg/d）1周,对照组给予生理盐水。伤后4周3组分别观测:①伤段脊髓残存组织面积;②采用斜板试验和运动功能评分观察大鼠后肢运动功能恢复情况。结果:大鼠脊髓损伤后4～14d,GDNF治疗组后肢运动功能评分明显高于NGF组和生理盐水对照组（P<0.05）。伤后28d GDNF组伤段残存脊髓组织面积大于对照组和NGF组（P<0.01）。结论:外源性GDNF能减少脊髓损伤后伤区的坏死、萎缩并促进大鼠后肢运动功能的早期恢复。

Effect of exogenous GDNF on motor function recovery following spinal cord injury in rats

Objective:The aim of the study is to investigate the effect of exogenous GDNF on spinal cord locomotor function recovery after spinal cord injury(SCI)in adult rats. Method:The SCI model of acute posterior spinal cord compression injury was established in rats according to the method of Nystrom. The recombinant rat GDNF or NGF was injected into the injured region upon intrathecal tubutization in first week after SCI, the control group instead of normal saline(NS). The aere of remaining injured spinal cord tissue was calculated by HE staining. The locomotion function of hindlimbs of rats was evaluated using inclined plane test and BBB locomotor scale. Result: The locomotion functional scales of rats in GDNF group were higher than that in NS group and NGF group within 4 to 14 days after SCI(P<0.05). Four weeks after SCI, the area of remaining injured spinal cord tissue in GDNF group was larger than that in NS group and NGF group (P< 0.01). Conclusion: These results indicate that exogenous GDNF may enhance locomotion functional restoration of hindlimbs as well as decreased tissue death and atrophy of lesion cord.