Evaluation of ochratoxin A for mutagenicity in a battery of bacterial and mammalian cell assays |
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| Authors: | A M Bendele S B Neal T J Oberly C Z Thompson B J Bewsey L E Hill M A Rexroat W W Carlton G S Probst |
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| Institution: | School of Veterinary Medicine, Purdue University, USA;Eli Lilly and Co., Greenfield, IN, USA;School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA;Eli Lilly & Co., Greenfield, IN 46140, USA |
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| Abstract: | Ochratoxin A (OA), a nephrotoxic mycotoxin, was evaluated for genotoxic potential in a battery of in vitro and in vivo assays. OA was not mutagenic to Salmonella typhimurium, either with or without metabolic activation, in the plate incorporation (Ames) test at concentrations of 50-600 micrograms OA/plate or in the gradient plate assay at concentrations of 0.1-1000 micrograms OA/ml. No induction of unscheduled DNA synthesis was evident in primary cultures of rat hepatocytes exposed to concentrations of OA ranging from 0.000025 to 500 micrograms/ml. In the mouse lymphoma forward mutation assay, exposure of L5178Y TK+/- mouse lymphoma cells to OA did not increase the numbers of L5178Y TK-/- mutants. There was no significant difference between the numbers of sister-chromatid exchanges in cells from OA-treated Chinese hamsters and those in cells from the negative-control animals. |
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| Keywords: | 2AA 2-aminoanthracene 2AAF 2-acetylaminofluorene 9AmAc 9-aminoacridine BUdR bromodeoxyuridine DMSO dimethylsulphoxide EMS ethyl methanesulphonate 3MCA 3-methylcholanthrene MNNG 2NF 2-nitrofluorene OA ochratoxin A OB ochratoxin B SCE sister-chromatid exchange TLC thin-layer chromatography To whom all correspondence should be addressed |
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