|
|||||
|
|
| 芫花根质量标准研究 | |
| 引用本文： | 韩伟,章军,吴立军,李娆娆. 芫花根质量标准研究[J]. 中国中医药信息杂志, 2009, 16(8): 45-46 |
| 作者姓名： | 韩伟 章军 吴立军 李娆娆 |
| 作者单位： | 1. 陕西中医学院药学院,陕西,咸阳,712046;中国中医科学院中药研究所,北京,100700 2. 中国中医科学院中药研究所,北京,100700 3. 陕西中医学院药学院,陕西,咸阳,712046;沈阳药科大学中药学院,辽宁,沈阳,110016 |
| 摘 要： | 目的建立芫花根中芫花酯甲的定性鉴别和定量分析方法。方法采用薄层色谱法对芫花根中芜花酯甲进行定性鉴别，采用高效液相色谱法测定芫花酯甲的含量。色谱条件：KromasilC18色谱柱（4．6mm×250mm，5μm）；流动相为甲醇-水（85：15），流速1．0mL／min；柱温40℃；检测波长为238nm；记录时间40min。结果芫花根中芫花酯甲的薄层色谱鉴别特征明显、专属性强；含量测定线性范围为0．047-0．282btg（r=0．9996）；平均回收率为99．2％，RSD=2．00％（n=6）。结论该方法操作简便，结果准确，能够对芜花根中的芜花酯甲进行定性鉴别和定量分析，提高了该药材的质量控制水平。 |
| 关 键 词： | 芫花根 芫花酯甲 薄层色谱法 高效液相色谱法 |
Research about Quality Standard on Root of Daphne genkwa Sieb.et Zucc |
|
| Affiliation: | HAN Wei, ZHANG Jun,WU Li-jun, et al (1.Pharmacy College, Shanxi University of TCM,, Xianyang 712046, China; 2.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; 3.School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China) |
| Abstract: | Objective To develop qualitative identification and quantitative analysis method of yuanhuacin in root of Daphne genkwa sieb.et Zucc. Method Yuanhuacin was identified by TLC and its content was determined by HPLC. Kromasil C18 （4.6 mm×250 mm, 5 μm） column was used. The mobile phase was methanol-water （85 ; 15） at a flow rate of 1.0 mL/min. The column temperature was 40 ℃. The detection wavelength of yuanhuacin were set at 238 nm. The record time was 40 min. Results The linear range of yuanhuacin was 0.047-0.282 μg （r =0.999 6）. The average recovery was 99.2% with RSD=2.0% （n ：6）. Conclusion These analytical methods were simple and accurate, and can be used for the qualitative identification and quantitative analysis of yuanhuacin. It is suitable for quality control of root of Daphne genkwa Sieb.et Zucc. |
| Keywords: | Daphne genkwa Sieb.et Zucc yuanhuacin TLC. HPIAE |
| 本文献已被 维普 万方数据 等数据库收录！ | |