| 高效液相色谱法测定小儿磨积片中橙皮苷的含量 |
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| 引用本文： | 苗淑杰,孙小容,尔凤君.高效液相色谱法测定小儿磨积片中橙皮苷的含量[J].中国药房,2005,16(20):1579-1580. |
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| 作者姓名： | 苗淑杰 孙小容 尔凤君 |
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| 作者单位： | 1. 天津同仁堂股份有限公司,天津市,300132
2. 太极集团有限公司,重庆市,400015 |
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| 摘 要： | 目的:建立以高效液相色谱法测定小儿磨积片中橙皮苷含量的方法。方法:色谱柱为SpherisorbC18,流动相为甲醇-冰醋酸-水(25∶4∶71),检测波长为283nm,流速为2·0ml/min,柱温为50℃,灵敏度为0·16AUFS,进样量为20μl。结果:橙皮苷进样量在0·024μg~1·2μg范围内与峰面积积分值呈良好的线性关系(r=0·9999),平均回收率为99·1%(RSD=0·8%)。结论:本方法简便、快捷,灵敏度及准确度高,可为小儿磨积片质量控制提供依据。
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| 关 键 词： | 高效液相色谱法 小儿磨积片 橙皮苷 含量 |
| 文章编号： | 1001-0408（2005）20-1579-02 |
| 收稿时间： | 2005-02-27 |
| 修稿时间： | 2005-05-01 |
Content Determination of Hesperidin in Xiaoermoji Tablets by HPLC |
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| MIAO Shujie,ER Fengjun,SUN Xiaorong.Content Determination of Hesperidin in Xiaoermoji Tablets by HPLC[J].China Pharmacy,2005,16(20):1579-1580. |
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| Authors: | MIAO Shujie ER Fengjun SUN Xiaorong |
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| Institution: | 1.Tianjin Tongrentang Limited Company, Tianj in 300132, China;2.Taiji Group,Chongqing 400015,China |
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| Abstract: | OBJECTIVE:To establish HPLC method for the determination of hesperidin in xiaoermoji tablets.METHODS:The determination was performed on Spherisorb C 18 ,the mobile phase consisted of methanol-glacial acetic acid-water(25∶4∶71),the detection wavelength was283nm,the flow rate was2.0ml/min,the column temperature was50℃,the sensitivity was0.16AUFS and the sample size was20μl.RESULTS:Hesperidin has a good linear relation with peak area score when its sample size was within a range of0.024μg～1.2μg(r=0.9999),the average recovery was99.1%(RSD=0.8%).CONCLUSION:The method is simple,fast,sensitive and accurate,and it can be served as a basis for the quality control of xiaoermoji tablets. |
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| Keywords: | HPLC Xiaoermoji tablets Hesperidin Contents |
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