姜黄素对戊四氮致癫大鼠海马区IL-2和IL-6表达水平的影响

目的探讨姜黄素抗癫的作用机制。方法取健康成年雄性SD大鼠,连续腹腔注射戊四氮,诱发大鼠点燃致癫模型。空白组和模型组灌予生理盐水5ml,1次/d,连续28d。低剂量和高剂量姜黄素组分别灌予姜黄素100mg/kg和200mg/kg,1次/d,连续28d;丙戊酸钠组灌予丙戊酸钠400mg/kg,1次/d,连续28d。治疗结束后,按照Racine的6级评分标准,观察癫大鼠发作等级变化,用酶联免疫法(ELISA)检测海马区IL-2和IL-6表达水平的变化。结果姜黄素组的癫大鼠惊厥发作等级降低。模型组IL-2和IL-6高表达,比空白组呈显著升高(P<0.05)。与模型组比较,姜黄素组大鼠海马区IL-2和IL-6的表达明显降低(P<0.05);与低剂量姜黄素组相比,高剂量姜黄素组IL-2和IL-6水平降低更为明显(P<0.05)。高剂量姜黄素组与丙戊酸钠组比较,无显著差异(P>0.05)。结论姜黄素能降低癫大鼠惊厥发作级别,具有一定的抗癫作用,并且呈剂量依赖性,其可能机制是通过降低海马区IL-2和IL-6的表达发挥作用。

Effect of the curcumin on expression of IL-2 and IL-6 of hippocampus in pentylenetetrazol-induced epilepsy in rats

Objective To investigate the mechanism of anti-epileptic effect of the curcumin. Methods The SD rats were injected intraperitoneally with pentylenetetrazol kindling 25.0 mg/kg to induce a rat epilepsy model. All of the treatments were performed once a day continuously for 28 days. The rats in blank group and model group received 5 ml of normal saline. The rats in the high and low curcumin group were given 200 mg/kg and 100 mg/kg of curcumin once a day, respectively. The rats in the sodium valproate (VPA) group were given 400 mg/kg of VPA once a day by gavage. After treatment, the seizures level was recorded by using the Racine''s six point grading scale, and the expression of IL-2 and IL-6 of hippocampus were detected by the enzyme linked immunoassay (ELISA). Results The seizures level was reduced by curcumin in epileptic rats. The expressions of IL-2 and IL-6 of the model group were significantly higher than those of the blank group (P<0.05), while those rats of the anti-epileptic groups, including high dose group and low dose group, were lower than those rats of the model group (P<0.05). When compared with the curcumin low dose group, the expression of IL-2 and IL-6 of curcumin high dose group is lower (P<0.05). There was no significant difference between the high dose curcumin group and VPA group (P>0.05). Conclusion The curcumin can reduce the seizure level in rats, it shows some anti-epileptic effets and dose-dependently, which may be through down-regulating the expression of IL-2 and IL-6 in hippocampus.