| UPLC法同时测定地锦草中4种指标成分的含量 |
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| 引用本文： | 冯光维,刘志东,祁东利,皮佳鑫,顾星,张谦,黄瑞. UPLC法同时测定地锦草中4种指标成分的含量[J]. 天津中医药大学学报, 2016, 35(3): 196-199 |
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| 作者姓名： | 冯光维 刘志东 祁东利 皮佳鑫 顾星 张谦 黄瑞 |
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| 作者单位： | 天津中医药大学 现代中药发现与制剂技术教育部工程研究中心, 天津 300193;黔南民族医学高等专科学校, 都匀 558000,天津中医药大学 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学 现代中药发现与制剂技术教育部工程研究中心, 天津 300193,天津中医药大学 现代中药发现与制剂技术教育部工程研究中心, 天津 300193 |
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| 基金项目： | 黔南民族医学高等专科学校科研基金资助项目（QNYZ2014014）。 |
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| 摘 要： | [目的]建立同时测定地锦草药材中没食子酸、鞣花酸、槲皮素、山奈酚4种指标成分的超高液相色谱法(UPLC)。[方法]采用UPLC法,色谱柱为Acquity UPLC BEH C18柱(100 mm×2.1 mm,1.7μm);流动相为甲醇-乙腈4∶6(A)-0.2%甲酸水(B)溶液,梯度洗脱:0~3 min,5%A;3~4 min,5%~17%A;4~19 min,17%A;19~42 min,17%~30%A;42~50 min,30%~34%A;50~55 min,34%~70%A,体积流量0.2 m L/min;检测波长为0~4 min,270 nm;4~55 min,360 nm,柱温30℃。[结果]4种成分均达到基线分离,各成分在线性范围内有良好的线性关系(r0.999 7);回收率在96.5%~102.9%。[结论]建立的测定方法准确灵敏、重复性好,能较全面地评价地锦草药材的质量。
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| 关 键 词： | UPLC 同时测定 地锦草 含量 |
| 收稿时间： | 2016-03-01 |
Simultaneous determination of four components in Euphorbia Humifusa Willd. by UPLC |
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| FENG Guang-wei,LIU Zhi-dong,QI Dong-li,PI Jia-xin,GU Xing,ZHANG Qian and HUANG Rui. Simultaneous determination of four components in Euphorbia Humifusa Willd. by UPLC[J]. Journal of Tianjin University of Traditonal Chinese Medicine, 2016, 35(3): 196-199 |
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| Authors: | FENG Guang-wei LIU Zhi-dong QI Dong-li PI Jia-xin GU Xing ZHANG Qian HUANG Rui |
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| Affiliation: | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Qiannan Medical College for Nationalities, Duyun, Guizhou, 558000, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China and Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract: | |
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| Keywords: | UPLC simultaneous determination Euphorbia Humifusa Willd. content |
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