白藜芦醇对胶原诱导大鼠关节炎的抗炎作用研究

目的 观察白藜芦醇对胶原诱导大鼠关节炎(CIA)的抗炎作用.方法 选择牛Ⅱ型胶原(CⅡ)为免疫原,建立CIA模型.选取42只造模成功的大鼠随机分为7组:模型组;来氟米特(LEF)对照组;白芍总苷(TGP)对照组;甲氨蝶呤(MTX)对照组;白藜芦醇(Res)小剂量组;白藜芦醇中剂量组;白藜芦醇高剂量组.采用关节炎指数评分(AI)法对大鼠关节炎症程度进行评分;采用酶联免疫吸附试验(ELISA)方法测定各组大鼠血清中抗CⅡ抗体水平.结果 AI评分:低剂量白藜芦醇组与模型组比较差异无统计学意义(12.3±0.5与12.8±0.4);高剂量白藜芦醇组低于中、低剂量组(6.0±0.6与8.2±1.0,12.3±0.5,P＜0.01).高剂量白藜芦醇组低于白芍总苷组(6.0±0.6与8.8±0.8,P＜0.01);LEF组低于TGP组、MTX组、3组白藜芦醇组(4.7±0.5与8.9±0.8,6.4+0.5,P＜0.01);血清抗CⅡ抗体水平:模型组明显高于LEF组、MTX组、TGP组及高、中剂量白藜芦醇组(0.928±0.021与0.391±0.016,0.503±0.010,0.525±0.015,0.507±0.01,0.570±0.021,P＜0.01),而与低剂量白藜芦醇组之间差异无统计学意义(0.928±0.021与0.908±0.026);高剂量白藜芦醇组低于白芍总苷组及低剂量白藜芦醇组(0.507±0.01与0.525±0.015,0.908±0.026,P＜0.01);LEF组低于高、中剂量白藜芦醇组(0.391±0.026与0.507±0.010,0.570±0.021,P＜0.01).结论 ①高、中剂量白藜芦醇能够缓解CIA的炎症症状,而低剂量白藜芦醇则无此作用.②中等剂量白藜芦醇对CIA大鼠的短期疗效与TGP相当,高剂量白藜芦醇的疗效强于TGP,但弱于LEF.③白藜芦醇能够抑制血清抗CⅡ抗体的生成.

The anti-inflammatory effect of Resveratrol on collagen-induced arthritis rats

Objective To investigate the anti-inflammatory effect of Resveratrol on type Ⅱ collagen induced arthritis.Methods Collagen induced arthritis (CIA) animal model was established by subcutaneous injection of type Ⅱ collagen emulsified with incomplete and complete Freud's adjuvant to Wistar rats.Fortytwo rats were successfully induced and randomly divided into 7 groups:the experimental group (A),the leflunomide treatment group (B),the TGP treatment group (C),the methotrexate group (D),the low dose Resveratrol group (E),the medium dose Resveratral group (F) and high dose Resveratrol group (G) and the normal control group (H).Symptoms of arthritis were recorded and selalm levels of the anti-C Ⅱ antibody were detected by ELISA.Results For arthritis index.there was no significant difference between groups E and A,neither between groups C and F.The arthritis index was lower in group G than group C,but both of them were higher than groups B and D.② For serum anti-C Ⅱ antibody level,that of group A was higher than groups B,C,D,F and G.There was no difference between groups A and E,and groups C and F.That of Group G was lower than groups C and E.Conclusion High and medium dose of Resveratrol can relieve foot joints swelling in the CIA rats,but low dose does not have similar effect.The effect of medium dose of Resveratrol iS similar to TGP,but weaker than that of leflunomide.Resveratrol may conduct its anti-inflammatory effect via lowering the concentration of the anti-C Ⅱ antibody in the serum.