流式细胞术快速鉴定ABO血型亚型

目的 建立流式细胞术快速鉴定ABO血型亚型的实验方法。方法 用荧光素(FITC)标记多人份人源抗-A、抗-B,与受检RBC上的相应抗原结合,利用流式细胞仪快速、特异地分辨每个细胞(生物颗粒)获得细胞的散点图,以RBC设定“门”,用Cell quest verl 2.2软件分析,对照管作“mark”,得测定管阳性细胞的百分率。结果 A型RBC其抗A-FITC百分率为97.40％±0.62％,抗B-FITC百分率为0.40％±0.15％;B型RBC其抗A-FITC百分率为0.42％±0.14％,抗B-FITC百分率为98.36％±0.55％。阴性细胞的x±s=0.43％±0.13％,阳性界值>0.85％(>x±3s)。11例ABO亚型FCM测定结果与血清学方法确认的结果完全一致。结论 流式细胞术鉴定ABO亚型的方法,较之常规的血清学方法,具有简便、快速、结果客观、重复性好之优点。

Rapid determination of ABO subgroups by flow cytometry

Objective To establish a new flow cytometry-based method to identify the subgroups of ABO. Methods Human source anti-A(B)labeled with FITC(anti-A(B)-FITC) were specifically bound to human RBC. The fluorescence dot plot diagrams of each cells were obtained by FCM. After gating RBC, the diagrams were analyzed by using software-cell quest verl 2.2.Results The anti-A-FITC and anti-B-FITC of blood group A RBC were 97.40% ± 0. 62% and 0. 40%±0.15% respectively. The anti-A-FITC and anti-B-FITC of blood group B RBC were 0. 42% ± 0.14% and 98. 36%±0.55%. The percentage of negative RBC was 0.43%±0. 13% and the threshold value was>0. 85%. FCM method showed consistent results with serologic testing for 11 cases of ABO subgroups. Conclusion FCM is more objective, sensitive, quicker and more precise than routine serologic typing method.