六味地黄汤对糖尿病大鼠心肌病变的影响

目的 通过研究六味地黄汤对糖尿病大鼠心肌细胞病理变化的影响,为其治疗糖尿病心脏并发症的药理作用提供实验依据.方法 随机将大鼠分为正常组、糖尿病组、六味地黄汤低、中、高剂量组(100、200和400mg.·kg-1·d-1)和罗格列酮组(100 mg·kg-1·d-1).除正常组外,其余各组采用腹腔注射链脲佐菌素法诱发糖尿病大鼠模型,造模成功后饲养4周,然后连续灌胃给药4周.于末次给药12 h后取材,观察大鼠心肌细胞的形态学变化及细胞凋亡指数.结果 与罗格列酮干预组相比,高剂量六味地黄汤能显著抑制糖尿病大鼠心肌细胞的形态学病变及细胞凋亡(P＜0.01),中剂量组亦具有一定抑制作用.结论 六味地黄汤对糖尿病心肌细胞的病理变化具有一定的抑制作用,可治疗该并发症.

Effects of Liuwei Dihuang Decoction on Myocardial Cell in Diabetic Rats

Objective To explore the effects of Liuwei Dihuang decoction on pathological myocardial cell in diabetic rats,and to provide experimental evidence for the pharmacological mechanism of Chinese medicine for diabetic cardiomyopathy.Methods Rats were randomly divided into control group,diabetic group,Liuwei Dihuang decoction low-dosage group(100 mg·kg-1·d-1),Liuwei Dihuang decoction mid-dosage group(200 mg·kg-1·d-1),Liuwei Dihuang decoction high-dosage group(400 mg·kg-1·d-1) and rosiglitazone group(100 mg·kg-1·d-1).Except normal group,rats in other groups were injected streptozotocin(4% STZ) intraperitoneally to induce diabetes,and bred for 4 weeks,then drugs were given after pathological myocardial cell in rats with diabetes occurred via Gastric gavage for 4 weeks consecutively.After this treatment,the electron microscopic sections of testis were made to observe the cell morphology and apoptosis of myocardium.Results Compared with the normal group,rats in the high dose group of decoction showed significant difference(P<0.01) in inhibition of myocardial lesions and apoptosis,and rats in the middle-dosage group had showed inhibition effects too.Conclusion Liuwei Dihuang decoction can inhibit pathological myocardial cell significantly,so it is effective on prevention and treatment of diabetic cardiomyopathy.