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| FGF-2对体外培养人牙髓细胞FGFR和OPN表达活性的影响 | |
| 引用本文： | 李桥,汪敏,韦永珍,孙虹,潘乙怀. FGF-2对体外培养人牙髓细胞FGFR和OPN表达活性的影响[J]. 牙体牙髓牙周病学杂志, 2011, 0(12): 675-678 |
| 作者姓名： | 李桥 汪敏 韦永珍 孙虹 潘乙怀 |
| 作者单位： | 温州医学院;黔西南州人民医院口腔科;温州医学院附属口腔医院; |
| 基金项目： | 浙江省教育厅科研基金资助项目(Y200907095); 浙江省大学生科技创新活动计划(新苗人才计划)资助项目(2011R413040) |
| 摘 要： | 目的:研究成纤维细胞生长因子-2(fibroblast growth factor-2,FGF-2)对体外培养的人牙髓细胞表面成纤维细胞生长因子受体(fibroblast growth factor receptor,FGFR)及骨桥蛋白(osteopontin,OPN)表达的影响。方法:以改良组织块培养法体外获得人牙髓细胞,采用Western blotting法检测不同浓度FGF-2作用下牙髓细胞FGFR的表达情况;应用Real Time-PCR法,检测不同浓度FGF-2作用下牙髓细胞OPN mRNA的表达。结果:与对照组相比,FGF-2在1～50 ng/mL浓度下均可促进牙髓细胞FGFR的表达(P<0.05),最佳显效浓度为10 ng/mL。FGF-2在1～50 ng/mL浓度下均可诱导牙髓细胞OPN mRNA表达(P<0.05),OPN mRNA的表达在10 ng/mL的FGF-2作用下达到高峰。结论:FGF-2可促进体外培养人牙髓细胞FGFR和OPN的表达,在牙髓牙本质复合体修复中可能发挥重要作用。 |
| 关 键 词： | 成纤维细胞生长因子-2 人牙髓细胞 成纤维细胞生长因子受体 骨桥蛋白 |
Effect of FGF-2 on FGFR and OPN expression of human dental pulp cells in vitro |
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| LI Qiao,WANG Min,WEI Yong-zhen,SUN Hong,PAN Yi-huai. Effect of FGF-2 on FGFR and OPN expression of human dental pulp cells in vitro[J]. Chinese Journal of Conservative Dentistry, 2011, 0(12): 675-678 | |
| Authors: | LI Qiao WANG Min WEI Yong-zhen SUN Hong PAN Yi-huai |
| Affiliation: | LI Qiao,WANG Min,WEI Yong-zhen,SUN Hong,PAN Yi-huai(Wenzhou Medical College,Wenzhou 325027,China) |
| Abstract: | AIM: To investigate the effect of fibroblast growth factor-2(FGF-2) on fibroblast growth factor receptor(FGFR) and osteopontin(OPN) expression of human dental pulp cells in vitro.METHODS: Human dental pulp cells were isolated from dental pulp tissue explants.FGFR expression of human dental pulp cells were examined by Western blotting assay.Real Time-PCR method was used to observe the OPN gene expression of human dental pulp cells.RESULTS: FGFR expression of human dental pulp cells was increased when FGF-2 w... |
| Keywords: | fibroblast growth factor-2 human dental pulp cells fibroblast growth factor receptor osteopontin |
| 本文献已被 CNKI 等数据库收录！ | |