氢氧化钙和洗必泰抑制粪肠球菌的抗菌性检测

目的:检测氢氧化钙和洗必泰凝胶根管封药对粪肠球菌的抑制作用。方法:将成功建立粪肠球菌感染的60个根管模型,随机分成4组,每组15个样本,分别用氢氧化钙、20 g/L洗必泰凝胶、氢氧化钙和20 g/L洗必泰凝胶联合、生理盐水(空白对照组)进行根管内封药处理,14 d后,分别用5号和6号GG钻钻取根管内壁层深0～100μm和100～200μm的牙本质,收集碎屑,用BHI梯度稀释后进行细菌培养和计数。结果:无论在根管壁深0～100μm处,还是100～200μm处,空白组均有大量的细菌生长,而3个实验组根管内粪肠球菌明显减少,分别与空白对照组相比,差异均具有统计学意义(P<0.05);各实验组之间相比,氢氧化钙组活菌数最高,与其他两组相比,差异均有统计学意义(P<0.05),其次为20 g/L洗必泰凝胶组,氢氧化钙联合洗必泰组最低,但与单独使用洗必泰组相比,差异无统计学意义(P>0.05)。结论:在根管封药中,氢氧化钙和洗必泰均对粪肠球菌有较好的抗菌性,其中洗必泰凝胶的抑菌作用更强。

Effect of calcium hydroxide and chlorhexidine against Enterococcus faecalis

AIM: To evaluate the efficacy of calcium hydroxide(Ca(OH)2) and chlorhexidine gel on the elimination of intratubular Enterococcus faecalis.METHODS: Sixty single-root teeth were infected with E.faecalis.The infected teeth were randomly divide into four groups,with 15 teeth in each group.The teeth were then treated with Ca(OH)2,20g/L chlorhexidine gel,Ca(OH)2 plus 20g/L chlorhexidine gel,or saline(0.9% NaCl) as a negative control.Dentin samples at the depth of 0 to 100 mm and 100 to 200 mm from these root can...