| RP-HPLC法同时测定橘叶中3个黄酮类成分的含量 |
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| 引用本文： | 汪金玉,帅欧,林励,卢惜香,谢崇芳,何彩萍. RP-HPLC法同时测定橘叶中3个黄酮类成分的含量[J]. 药物分析杂志, 2011, 0(11) |
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| 作者姓名： | 汪金玉 帅欧 林励 卢惜香 谢崇芳 何彩萍 |
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| 作者单位： | 广州中医药大学中药学院; |
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| 摘 要： | 目的:建立同时测定橘叶中川陈皮素、橘红素、5-去甲川陈皮素含量的反相高效液相色谱法。方法:采用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-乙腈-水(体积比30∶30∶40),流速1.0 mL.min-1,检测波长342 nm;柱温为室温(25℃)。结果:川陈皮素、橘红素、5-去甲川陈皮素线性范围分别为0.239～2.870μg(r=0.9995),0.102～1.224μg(r=0.9995),0.054～0.643μg(r=0.9995);平均回收率(n=6)分别为98.06%,98.02%,97.61%,RSD分别为2.6%,1.9%,2.0%。结论:本法快速、简便、准确,重复性好,可用于橘叶川陈皮素、橘红素、5-去甲川陈皮素的定量分析和质量控制。
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| 关 键 词： | 反相高效液相色谱法 橘叶 黄酮类 川陈皮素 橘红素 5-去甲川陈皮素 |
RP-HPLC simultaneous determination of three flavonoid components in Folium Citri Reticulatae |
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| WANG Jin-yu,SHUAI Ou,LIN Li,LU Xi-xiang,XIE Chong-fang,HE Cai-ping. RP-HPLC simultaneous determination of three flavonoid components in Folium Citri Reticulatae[J]. Chinese Journal of Pharmaceutical Analysis, 2011, 0(11) |
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| Authors: | WANG Jin-yu SHUAI Ou LIN Li LU Xi-xiang XIE Chong-fang HE Cai-ping |
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| Affiliation: | WANG Jin-yu,SHUAI Ou,LIN Li,LU Xi-xiang,XIE Chong-fang,HE Cai-ping (College of Chinese Materia Medica,Guangzhou University of Chinese Medicine,Guangzhou 510006,China) |
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| Abstract: | |
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| Keywords: | RP-HPLC Folium Citri Reticulatae nobiletin flavone tangeretin 5-O-desmethyl nobiletin |
| 本文献已被 CNKI 等数据库收录！ |
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