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| 高效液相色谱测定维酶素中的核黄素 | |
| 引用本文： | 刘伟 薛顺国. 高效液相色谱测定维酶素中的核黄素[J]. 中国医药工业杂志, 1990, 21(10): 455-456 |
| 作者姓名： | 刘伟 薛顺国 |
| 作者单位： | 襄樊市药品检验所，襄樊市药品检验所 湖北 441021，湖北 441021 |
| 摘 要： | 高效液相色谱法测定维酶素中核黄素,利用十八烷基键合相柱,以pH4.5的甲醇-醋酸缓冲液(33:66)为流动相,流速0.5ml/min,以对乙酰氨基酚为内标,检测波长267nm,平均回收率101.1％,变异系数0.72％,此法重现性好。 |
| 关 键 词： | 维酶素 核黄素 高效液相色谱 |
HPLC DETERMINATION OF RIBOFLAVIN IN WEIMESU |
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| LIU Wei,XUE Shun-Guo Xiangfan Institute for Drug Control,Hubei. HPLC DETERMINATION OF RIBOFLAVIN IN WEIMESU[J]. , 1990, 21(10): 455-456 | |
| Authors: | LIU Wei XUE Shun-Guo Xiangfan Institute for Drug Control Hubei |
| Affiliation: | LIU Wei,XUE Shun-Guo Xiangfan Institute for Drug Control,Hubei 441021 |
| Abstract: | A reversed-phase HPLC is described for determining riboflavinin Weimesu with Spheri-5 RP-18 column. The mobile phase is pH 4.5 methanol-acetic acid buffer (33:66). Paracetamol is chosen as internal standard, monitoredby UV detector at 267 nm. The recovery of riboflavin is 101.1% and coefficientof variation is 0.72%. |
| Keywords: | Weimesu riboflavin HPLC |
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