动脉硬化闭塞症与内皮素、一氧化氮的关系

目的 探讨内皮素（ＥＴ）、一氧化氮（ＮＯ）在动脉硬化闭塞症（ＡＳＯ）形成和发展听 作用及ＥＴ、一氧化氮合酶（ＮＯＳ）基因表达同ＡＳＯ的关系。方法 收集４０例ＡＳＯ病人、１２０处病变血管和３０例正常血管，根据病理学检查分为ＡＳＯ早期、中期、晚期和正常血管４组。利用放射免疫测定法和Ｇｒｉｓｓｅ方法检测ＡＳＯ病脸的血浆ＥＴ和ＮＯ水平，利用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）技术检测４组血管ＥＴｍＲＮＡＴ

Relationship between atherosclerosis obliterans and endothelins, nitric oxide

Objective To investigate the role of plasma endothelins (ET), nitric oxide (NO) in the pathogenesis of atherosclerosis (AS) obliterans (ASO) and the relationship between ASO and ET, NO synthase (NOS) gene expression. Methods 120 samples from 40 patients with ASO were collected and 30 samples of normal arteries from traumatic amputation and fresh cadavers. All the samples were divided into early, middle and late stage ASO group and normal control group according to pathology. The plasma ET and NO levels were determined by radioimmunoassay and Griss method in the ASO patients. The ET mRNA and NOS mRNA gene expression in the four groups was detected by RT PCR method. Results The ASO patients showed high level of plasma ET and low level of plasma NO. The ASO artery NOS enzyme activity and NOS mRNA expression were decreased significantly, while the ET mRNA gene expression increased obviously, all changes were found most obviously in the advanced ASO arteries.Conclusion The high ET expression and low NOS mRNA expression existed during the development of ASO. High ET level and low NO level occurred in the process of ASO pathogenesis.