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| 耐左氧氟沙星结核分枝杆菌gyrA基因上新的突变位点 | |
| 引用本文： | 尹小毛,刘志辉,黄丽晶,蔡杏珊,罗春明. 耐左氧氟沙星结核分枝杆菌gyrA基因上新的突变位点[J]. 实用医学杂志, 2010, 26(5). DOI: 10.3969/j.issn.1006.5725.2010.05.008 |
| 作者姓名： | 尹小毛 刘志辉 黄丽晶 蔡杏珊 罗春明 |
| 作者单位： | 1. 广州医学院 2. 广州市胸科医院肺研室,510095 |
| 基金项目： | 广州市医药卫生科技项目 |
| 摘 要： | 目的:分析耐左氧氟沙星结核分枝杆菌临床菌株gyrA基因的突变情况及其耐药机制.方法:用聚合酶链反应(PER)和DNA直接测序技术(DS)测定64株耐左氧氟沙星结核分枝杆菌gyrA基因的QRDR(quinolone resistance-determining regions)序列.结果:64株耐药菌株有47株QRDR序列发生突变,其中45株为单位点突变,另2株为双位点突变;突变分布为70位突变2株、89位1株、90位12株、91位4株、94位30株,其中70位和89位为新发现的突变位点.结论:结核分枝杆菌喹诺酮类药物耐受现象与gyrA基因QRDR的突变有关,包括新发现的70位和89位突变. |
| 关 键 词： | 结核 喹诺酮 结核分枝杆菌 gyrA基因 |
New mutations of gyrA gene in levofloxacin-resistant Mycobacterium tuberculosis clinical isolates |
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| YIN Xiao-mao,LIU Zhi-hui,HUANG Li-jing,CAI Xing-shan,LUO Chun-ming. New mutations of gyrA gene in levofloxacin-resistant Mycobacterium tuberculosis clinical isolates[J]. The Journal of Practical Medicine, 2010, 26(5). DOI: 10.3969/j.issn.1006.5725.2010.05.008 | |
| Authors: | YIN Xiao-mao LIU Zhi-hui HUANG Li-jing CAI Xing-shan LUO Chun-ming |
| Affiliation: | YIN Xiao-mao,LIU Zhi-hui,HUANG Li-jing,CAI Xing-shan,LUO Chun-ming.Institute for Pulmonary Disease,Guangzhou Chest Hospital,Guangzhou 510095,China |
| Abstract: | Objective To observe the mutations of gyrA gene in levofloxacin-resistant Mycobacterium Tuberculosis(M.tuberculosis) clinical isolates and study the molecular mechanism of these mutations.Methods Quinolone resistance-determining regions of gyrA gene in sixty-four levofloxacin-resistant M.tuberculosis clinical isolates were analyzed with polymerase chain reaction and DNA direct-sequencing.Results Forty-five of sixty-four clinical isolates mutated in one codon of gyrA gene and two in double codons.Two isolate... |
| Keywords: | Tuberculosis Quinolones Mycobacterium tuberculosis gyrA gene |
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