实验性大鼠脑出血后细胞凋亡与蛋白激酶C同工酶表达的关系及水蛭素的干预作用

目的探讨大鼠脑出血后蛋白激酶C同工酶表达及细胞凋亡程度,以及水蛭素对二者的影响。方法采用未抗凝新鲜自体股动脉血注入大鼠尾状核建立脑出血动物模型,75只大鼠随机分为对照组、脑出血组及水蛭素组。用免疫组织化学法及原位末端标记法分别测定脑出血后6 h、1天、3天、6天、10天等时间点血肿周围组织蛋白激酶C同工酶的表达和细胞凋亡程度,以及水蛭素对二者的影响。结果与对照组比较,血肿周围组织中蛋白激酶C同工酶水平及凋亡细胞数在脑出血后6 h升高(P<0.05),第3天达高峰(P<0.001),此后下降,10天时的表达接近正常,差异无显著性(P>0.05);水蛭素能够降低各个时间点蛋白激酶C同工酶水平及凋亡细胞的表达。结论脑出血后血肿周围蛋白激酶C同工酶水平及凋亡细胞的表达均上调,而水蛭素能够抑制细胞凋亡,其机制可能与抑制蛋白激酶C同工酶的表达有关。

The Relationship Between the Expression of Protein Kinase C Isoenzyme and Apoptosis after Intracerebral Hemorrhage in Rats,and the Intervention Effect of Hirudin

Aim To investigate the expression of protein kinase C(PKC) isoenzyme and apoptosis in rats following intralcerebral hemorrhage,and the intervention effect of hirudin.Methods Animal model was made by injecting self arterial blood into the caudate nucleus of rats.75 rats were randomly assigned to control group,cerebral hemorrhage group and aprotinin treated group.After the injection,the rats were sacrificed at 6 h,1 d,3 d,6 d,10 d respectively.The expression of PKC isoenzyme was measured by immunohistochemistry method,and apoptosis detected with TUNEL method over a time course ranging from 6 hours to 10 days.Results Compared with the control group,the level of PKC isoenzyme increased after 6 hours of intralcerebral hemorrhage and reached its climax at the 3 day,then decreased at the 6 day and come back to the normal standard at the 10 day.Administration of aprotinin could reduce the expression and the level of apoptosis.Conclusions The expression of protein kinase C isoenzyme and apoptosis were up-regulated in rats following intralcerebral hemorrhage;Aprotinin can reduce the level of apoptosis;the inhibited expression of PKC isoenzyme may be involved in the mechanisms.