哮喘豚鼠气道中血管内皮细胞生长因子和内皮抑素动态变化对气道重塑的影响

目的探讨哮喘豚鼠气道中血管内皮细胞生长因子（VEGF）和内皮抑素（ES）动态变化对气道重塑的作用。方法将40只豚鼠随机分为正常对照组（C组）、生理盐水组（N组）、哮喘3d组（A1组）、哮喘7d组（A2组）和哮喘21d组（A3组）,8只／组。C组豚鼠不行任何处理。N组分别于1d和8d腹腔注射生理盐水2ml,15d开始用37℃生理盐水雾化吸入,1次／d,3～5min／次,共3d;A1、A2和A3组用卵蛋白（OVA）10mg、氢氧化铝20mg制成2ml悬液代替生理盐水作腹腔注射,用1％OVA代替生理盐水分别水雾化吸入3d、7d和21d,其他方法同N组。末次激发24h后麻醉行支气管肺泡灌洗,收集支气管肺泡灌洗液（BALF）用酶联免疫（ELISA）法测VEGF和ES浓度。用图像分析仪测定气道内周径（Pi）,外周径（Pe）,并计算管壁面积（WA）,气道平滑肌面积（SMC—A）,气道平滑肌细胞数（N）,且WA、SMC—A、N均用Pi进行标准化。结果哮喘豚鼠随病程延长,WA／Pi、SMC-A／Pi、N／Pi和气道中VEGF浓度进行性升高,与C组和N组比较,哮喘各组中SMC-A／Pi、N／Pi和VEGF浓度差异有统计学意义,A,和A,组中WA／Pi差异有统计学意义。哮喘豚鼠气道中VEGF浓度与wA／H呈正相关（r=0．51,P〈0．05）,VEGF／ES与WA／Pi呈正相关（r=0．78,P〈0．01）。结论VEGF／ES平衡失调可能在哮喘豚鼠气道重塑起重要作用。

Dynamic changes of vascular endothelial growth factor and endostatin in the airway and its effect on airway remodeling in asthma guinea-pig

Objective To investigate dynamic changes of vascular endothelial growth factor(VEGF)and endostatin(ES)in guinea.Pig of asthma and its effect on airway remodeling.Methods 40 guinea-pigs were randomly divided into normal control group(C),NS group(N),3d asthma group(A1),7d asthma group(A2)and 21d asthma group(A3),8 pigs ofeach group·Nothing was done on C Group guinea.Pigs.N group were intraperitoneal injected 2ml NS on the Ist day and the 8th day.NS inhalation started on the 15th day,1 time every day,3-5rain for each times,lasted for 3 day.Al,A2 and A3 Group were injected with 10rag ovalbumin(OVA),which made of aluminum hydroxide 20mg suspension in place of2ml NS,in place ofNS inhalation for3d,7d and 21d.At the end of24h-shot line after anesthesia BAL.Enzynle-linked immunosorbent(ELISA)was used to determine the concentration of VEGF and ES in bronchoalveolar lavnge fluid collection(BALF).Image analysis was used to determine airway circumference(Pi),outer circumference(Pe),and calculate the wall area(WA),airway smooth muscle area(SMC-A),airway smooth muscle cells(N).WA,SMC-A and N were standardized by Pi.Results With the extension of the duration of asthmatic guinea.Pigs,WA/Pi,SMC-A/Pi,N/Pi and concentration of VEGF in the airway were increased.Compared with group C and N,SMC-A/Pi,N/Pi and concentration of VEGF in asthma group were siguitlcanfly different.WA/Pi were siguificanfly different in A2 group and A3 group.The relation of the concentration of VEGF in Asthma guinea-pigs airway and WA/Pi was positive correlation.VEGF/ES and WA/Pi was highly positive correlated.Conclusions VEGF/ES inlbalance may play all important role in the airway remodeling of asthmatic guinea-pigs.