电纺漏斗网蜘蛛丝纤维性能及体外细胞生物学性能

目的：近年来，蜘蛛丝在基因识别、人工合成以及基因表达上取得的成果，引发了广大研究者模拟天然蜘蛛丝优秀性能的兴趣。采用静电纺技术对制备的漏斗网蜘蛛丝再生纤维膜进行性能检测，为静电纺蜘蛛丝纳米纤维应用于组织工程和生物医学领域提供前期研究。方法：实验于2006—07／2007—03在东华大学生物研究所生物材料室完成。采用电纺技术制备了漏斗网蛛丝再生纤维膜，对纤维膜的表面形态分析，水解性能，热性能，力学性能进行检测。体外与猪动脉内皮细胞共培养，采用MTT法检测对纤维膜细胞的增殖活性，以相差显微镜和扫描电镜观察细胞的形态变化。结果：再生蛛丝纤维膜热分解起始温度为279℃；在单轴拉伸时断裂强度和断裂伸长率分别为（3．61±0．18）MPa和（33．20±4．86）％；内皮细胞能够在纤维表面黏附并显示良好的生长形态；MTT结果显示内皮细胞在材料上增殖活跃，培养7d后，纤维膜上的细胞增殖为对照组的两倍多。结论：漏斗网蜘蛛丝再生纤维膜显示出稳定的热性能和高的延展性，并能有效促进内皮细胞的黏附和增殖，具育良好的生物相容性。

Property and biocompatibility of electrospun funnel-web spider silk fibrous mats

AIM： Spider silk shows good properties in gene identification, artificial synthesis and gene expression, which bring high interests of investigators to stimulate natural spinal silk. This paper introduces the preparation of electrospun funnel-web spider silk fibrous mats and provides basis for the application of nanofibrous mats of electrospun spider silk in tissue engineering and biomedicine. METHODS： Experiments were performed at the Room of Biomaterial of Institute of Biology of Donghua University from July 2006 to March 2007. Nanofibrous mats of funnel-web spider silks were electrospun. The surface of fibrous mats was analyzed. Hydrolysis, thermal properties and mechanical properties of resulted fibers were investigated. Resulted fibers were cocultured with endothelial cells of porcine artery in vitro. The cell proliferation and cell morphologies were respectively evaluated by MTF method and phase contrast microscope and scanning electron microscope （SEM）. RESULTS： The elelctrospun spider silk mats exhibited a thermal stability below 279℃. The breaking stress was （3.61 ± 0.18） MPa and the breaking strain was （33.20 ± 4.86）%. Endothelial cells grew well and could adhere to the surface of fibers. MTF tests showed that endothelial cells proliferated actively. The number of cells on fibrous mats was as twice as that in the control group seven days later. CONCLUSION： Nanofibrous mats of Funnel-web spider silks process stable thermal properties and high ductility. Electrospun mats exhibit good biocompatibility and can enhance the adhesion and proliferation of endothelial cells.