| 异氟醚后处理对盲肠结扎穿孔大鼠急性肺损伤的保护作用 |
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| 引用本文： | 徐文莉,何俊,曾因明,庞庆丰.异氟醚后处理对盲肠结扎穿孔大鼠急性肺损伤的保护作用[J].徐州医学院学报,2010,30(5):290-293. |
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| 作者姓名： | 徐文莉 何俊 曾因明 庞庆丰 |
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| 作者单位： | 1. 江苏省麻醉与镇痛应用技术重点实验室,江苏,徐州,221002
2. 江苏省麻醉与镇痛应用技术重点实验室,江苏,徐州,221002;徐州医学院病理生理学教研室,江苏,徐州,221002 |
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| 基金项目： | 江苏省研究生培养创新工程，徐州医学院院长人才专项基金 |
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| 摘 要： | 目的观察异氟醚（isoflurane,ISO）后处理对盲肠结扎穿孔术（cecal ligation and puncture,CLP）后大鼠急性肺损伤（acute lung injury,ALI）的保护作用。方法32只SD雄性大鼠随机分为4组（每组n=8）：正常组（N组）,未行任何处理;假手术组（Sham组）,单纯行开腹关腹术;盲肠结扎穿孔术组（CLP组）,行CLP建立大鼠ALI模型;异氟醚组（ISO组）,建立CLP模型后4 h腹腔注射液态异氟醚2 ml/kg。术后12 h各组动物腹主动脉放血处死,右肺进行病理切片和肺湿/干比（W/D）测定,组织匀浆测超氧化物歧化酶（SOD）、丙二醛（MDA）和髓过氧化物酶（MPO）的含量;左肺行支气管肺泡灌洗,取上清液测总蛋白、肿瘤坏死因子α（TNF-α）和白细胞介素10（IL-10）的含量。结果肺组织病理学检测结果显示CLP组大鼠肺组织损伤严重,ISO组肺组织损伤较轻。与N组比较,CLP组和ISO组中SOD含量降低（P〈0.01）,其余检测指标均增加（P〈0.01）。与CLP组比较,ISO组SOD和IL-10含量较高（P〈0.01）;其余检测指标均低于CLP组（P〈0.05或P〈0.01）。结论在本实验条件下,大鼠腹腔注射液态ISO可发挥抗炎、抗氧化作用,对CLP所致大鼠ALI有部分保护作用。
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| 关 键 词： | 急性肺损伤 肺保护 异氟醚 后处理 盲肠结扎穿孔 |
The protective effect of isoflurane post-treatment on the acute lung injury induced by cecal ligation and puncture in rats |
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| XU Wenli,HE Jun,ZENG Yinming,PANG Qingfeng.The protective effect of isoflurane post-treatment on the acute lung injury induced by cecal ligation and puncture in rats[J].Acta Academiae Medicinae Xuzhou,2010,30(5):290-293. |
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| Authors: | XU Wenli HE Jun ZENG Yinming PANG Qingfeng |
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| Institution: | XU Wenli1,HE Jun1,ZENG Yinming1,PANG Qingfeng1,2(1.Jiangsu Province Key Laboratory of Anesthetic , Analgesic Application Technology,Xuzhou,Jiangsu 221002,China,2.Department of Pathophysiology,Xuzhou Medical College,Jiangsu 221002) |
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| Abstract: | Objective To investigate the protective effects of isoflurane(ISO) post-treatment on acute lung injury(ALI) induced by cecal ligation and puncture(CLP) in rats.Methods 32 male Sprague-Dawley(SD) rats were randomly divided into 4 groups(n=8 each): normal(N) group,with no treatment;Sham-operated control(Sham) group,with mere incision and closure of the abdomen;cecal ligation and puncture(CLP) group,established as rat model of induced ALI;and isoflurane(ISO) group,with intraperitoneal injection of 2 ml/kg ISO ... |
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| Keywords: | acute lung injury lung protection isoflurane post-treatment cecal ligation and puncture |
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