邻苯二甲酸二丁酯和二(2-乙基已基)酯对大鼠尿液中超氧化物歧化酶酶活力和丙二醛含量的影响

目的探讨邻苯二甲酸二丁酯(DBP)和邻苯二甲酸二(2-乙基己基)酯(DEHP)一次性单独及一次性联合染毒对雄性SD大鼠尿液超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量影响。方法选取60只体质量200 g左右SD雄性大鼠,测量其体质量并随机分为10组,分别是阴性对照组(芝麻油)、DBP单独染毒组(低剂量:30 mg/kg;中剂量:100 mg/kg;高剂量:300 mg/kg)、DEHP单独染毒组(低剂量:50 mg/kg;中剂量:150 mg/kg;高剂量:450 mg/kg)以及DBP和DEHP联合染毒组(低剂量:DBP 30mg/kg+DEHP 50 mg/kg;中剂量:DBP 1 00 mg/kg+DEHP 1 50 mg/kg;高剂量:DBP 300 mg/kg+DEHP 450 mg/kg),每组6只。采用灌胃的方式进行一次性染毒,灌胃量为2 ml。染毒后收集24、48、72、96 h尿液,并测定其尿液中SOD酶活性和MDA含量。结果 DBP、DEHP单独和联合染毒均能在不同程度上降低大鼠24 h尿液中SOD酶活力,增加24 h尿液中MDA含量,但对大鼠48、62、96 h尿液中SOD酶活力和MDA含量的影响则无显著性差异;各组大鼠随着饲养天数的增加尿液中SOD酶活力有所回升,MDA含量有所下降。结论 DBP、DEHP单独和联合染毒均能在短期内对大鼠肾脏造成显著的氧化损伤,且联合染毒效应更高。

Effect of dibutyl phthalate and di-(2-ethylhexyl) phthalate on urine SOD activity and MDA content in rats

Objective To evaluate the effect of dibutyl phthalate(DBP) and di-(2-ethylhexyl) phthalate(DEHP) on urine superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in rats.Methods According to 2×2 factorial analysis,60 adult male SD rats were randomized into 10 groups(n=6),including a control group(fed with sesame oil),3 DBP groups(fed with DBP at the doses of 30,100 and 300 mg/kg),3 DEHP groups(with DEHP at 50,150,and 450 mg/kg),and 3 DBP+DEHP groups(with 30 mg/kg DBP+50 mg/kg DEHP,100 mg/kg DBP+150 mg/kg DEHP,and 300 mg/kg DBP +450 mg/kg DEHP).The agents were administered in a single dose through gavage in a volume of 2 ml.After the treatments,the 24,48,72, and 96 h urine samples were collected to determine the SOD activity and MDA content.Results DBP and DEHP,either alone or in combination,significantly decreased SOD activity and increased MDA content in the urine collected at 24 h but not at the other time points.Such changes were gradually reversed with time.Conclusion DBP or DEHP treatment alone can result in significant oxidative damage in the kidney of rats,and the toxic effect of the combined exposure is even more obvious.