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| 副肿瘤性天疱疮患者免疫荧光与免疫印迹检测研究 | |
| 引用本文： | 李丽,朱学骏,卜定方,秦俭,陈喜雪. 副肿瘤性天疱疮患者免疫荧光与免疫印迹检测研究[J]. 中华皮肤科杂志, 2002, 35(4): 281-283 |
| 作者姓名： | 李丽 朱学骏 卜定方 秦俭 陈喜雪 |
| 作者单位： | 100034,北京大学第一医院皮肤性病科 |
| 摘 要： | 目的 研究副肿瘤性天疱疮患者的免疫学特点。方法 采用免疫荧光和免疫印迹方法检测4例副肿瘤性天疱疮患者肿瘤切除前、后的血清。结果 以鼠膀胱为底物行间接免疫荧光示IgG和C3棘细胞间沉积，肿瘤切除后抗体滴度较术前下降，且与病情的好转成正比。比正常人皮肤及大鼠舌、食管及猴舌为底物行间接免疫荧光阳性；以大鼠支气管、心肌、肝脏、肾脏等组织为底物行间接免疫荧光阴性。免疫印迹示患者血清可识别人角质形成细胞提取物中的210000和190000抗原。结论 以鼠膀胱为底物的间接免疫荧光可作为副肿瘤性天疱疮的过筛试验，且可通过抗体滴度的改变监测病情的变化。副肿瘤性天疱疮更易累及粘膜部位上皮组织。免疫印迹结果可确诊4例患者为副肿瘤性天疱疮。 |
| 关 键 词： | 副肿瘤性天疱疮 间接荧光抗体技术 免疫印迹法 PNP 免疫学 |
| 修稿时间： | 2001-08-04 |
Immunofluorescent and Immunoblotting Features of Paraneoplastic Pemphigus |
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| LI Li,ZHUXuejun,BU Dingfang,QIN Jian,CHEN Xixue.Depa rtment of Dermatology,First Hospit al,Peking University,Beijing,China. Immunofluorescent and Immunoblotting Features of Paraneoplastic Pemphigus[J]. Chinese Journal of Dermatology, 2002, 35(4): 281-283 | |
| Authors: | LI Li ZHUXuejun BU Dingfang QIN Jian CHEN Xixue.Depa rtment of Dermatology First Hospit al Peking University Beijing China |
| Affiliation: | LI Li,ZHUXuejun,BU Dingfang,QIN Jian,CHEN Xixue.Depa rtment of Dermatology,First Hospit al,Peking University,Beijing100034,China |
| Abstract: | Objective To investigate the immunofluoresce nt and immunoblotting features of paraneoplastic pemphigus(PNP).Methods Sera were tested by indirect immunofluorecence(IIF)and immunoblotting(IB)in four patients with PNP.Results Binding of IgGand C3to the intercell ular spaces of epidermis was found in IIF with rat bladder as substrate.The titers decreased after the excision of tumo rs.The autoantibodies also bound to sub-strates of the epithelia of human ski n,rat tongue and esophagus in those p atients.The patients' sera recogni zed 210kD and 190kD antigens from human kera tinocyte extracts with IB analysis.Conclusions IIF with rat bladder as substrate can be used a screening test for PNP.PNP tends to involve epithelia of mucosa.The results of IB test m ay confirm the diagnosis of PNP of the 4p atients.[ |
| Keywords: | Pemphigus paraneoplastic Fluorescent antib ody technique indirect Immunoblo tting |
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