依降钙素对破骨细胞的作用观察

目的 观察了国产降钙素-依降钙素对破骨细胞体外骨吸收功能的影响。并与进口降钙素-益钙宁的作用比较，方法 由10日龄幼兔四肢长骨机械分离破骨细胞于199培养液（含10%NCS 5?S），分别接种于象牙薄切片和培养板，置37℃，5%CO2培养箱中培养，细胞骨架观察采用荧光标记法（罗达明标记的鬼笔环肽），骨片吸收陷窝计数采用甲苯胺蓝染色法，益钙宁为阳性对照，等量培养液为阴性对照，结果 依降钙素组破骨细胞F-actin聚集，变短，骨片培养3d，吸收陷窝计数的结果显示，10^-10mol/L以上浓度依降钙素对破骨细胞吸收功能有明显抑制作用，并呈剂量相关性，10^-1mol/L时的抑制作用高达90%；依降钙素和益钙宁的抑制作用呈高度相关（r=0.99）。结论 依降钙素具有明显的抑制体外培养破骨细胞骨吸收活性的作用，与益钙宁的作用相仿。

Effects of Yijianggaisu on osteoclasts in vitro

Objective\ To evaluate the pharmacodynamic effects of Yijianggaisu, a new brand Chinese made calcitonin, on osteoclasts in vitro. Methods\ The osteoclasts isolated from long bones of 10 day old rabbits by curette and agitation were cultured on dentin slices or in plastic dishes with and without Yijianggaisu. The F actin distribution in cells, marked by rhodamine phalloidin, was observed and the resorption pits on slices, stained by toluidine blue, were counted. Elcatonin was used as a positive control. Results\ The patterns of osteoclastic F actin became aggregated and shortened by Yijianggaisu. The pits excavated by osteoclasts decreased in Yijianggaisu above 10 -10 mol/L and the inhibitory effect was correlated ( r =0 99) with that of Elcatonin in the same dosage. Conclusion\ Yijianggaisu, an analogue to Elcatonin, is effective in inhibiting osteoclast resorption in vitro.