头颈肿瘤组织标本库构建的初步研究

目的 探讨头颈肿瘤组织标本的采集、保存，组织库构建方法，以及信息化管理技术。方法 收集手术切除和活检的头颈肿瘤组织、癌旁组织、远端正常组织及患者和正常人外周血标本(分离出血清、血浆、淋巴细胞、干细胞等)，置于液氮或-80℃低温冰箱冷冻保存；同时建立一套系统管理组织标本库，使标本库规模化、信息化、智能化。结果 共收集新鲜组织标本220份，血液标本476份。其中肿瘤组织120份(包括肿瘤组织、癌旁组织)；非肿瘤组织100份。肿瘤患者血液标本316份，非肿瘤患者血液标本160份。结论 标本库的建立使少见的头颈肿瘤组织标本集约化、规模化，极大提高了标本利用的效率，达到了资源共享的目的。

A preliminary study to establish the tumor bank for head and neck carcinoma

Objective To explore the methods to establish a tumor bank for head and neck carcinoma by collecting and preserving the tumor tissue specimens with an information-based database. Methods The tumor tissues, tumor-adjacent tissues, normal tissues were collected from surgery or biopsy, as well as peripheral blood samples, including serum, plasma, lymphocyte or stem cells, etc, from head and neck carcinoma patients and healthy subjects. All the specimens were stored in liquid nitrogen or -80℃ refrigerator. Meanwhile, an information-based database was established. Results Specimens from 220 cases were collected, including 120 cases of carcinoma (tumor and tumor-adjacent tissues) and 100 cases of tissues without tumor. Samples of peripheral blood from 476 cases were collected, including 316 cases of carcinoma and 160 healthy subjects. Conclusion The tissue bank of head and neck carcinoma can be established large-scalely and intensively, which can greatly enhance the efficiency of specimen usage.