| 人表皮生长因子真核表达载体的构建 |
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| 引用本文： | 赵国强,张立华,王莉,鲍玉洲. 人表皮生长因子真核表达载体的构建[J]. 郑州大学学报(医学版), 2004, 39(4): 603-605 |
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| 作者姓名： | 赵国强 张立华 王莉 鲍玉洲 |
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| 作者单位： | 郑州大学基础医学院微生物学与免疫学教研室,郑州,450052;河南省人民医院,河南省眼科研究所分子生物室,郑州,450003 |
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| 基金项目： | 河南省科研事业发展计划项目 0 14 1163 2 16 |
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| 摘 要： | 目的：构建人表皮生长因子的真核表达载体。方法：采用RT-PCR扩增人表皮生长因子(hEGF)cDNA基因序列并克隆入PGEM-T载体，再用限制性内切酶切取目的基因，插入pcDNA3．1真核表达载体。结果：重组体经转化E．coli JM109感受态大肠杆菌后可大量扩增，提取重组体进行限制性酶切及PCR可见目的片段。结论：已成功构建hEGF真核表达载体，为进一步研究hEGF的功能奠定了基础。
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| 关 键 词： | 人表皮生长因子 克隆 基因表达 |
| 修稿时间： | 2003-09-04 |
Construction of eukaryotic expression vector for human epidermal growth factor |
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| ZHAO Guoqiang ),ZHANG Lihua ),WANG Li ),BAO Yuzhou ) ). Construction of eukaryotic expression vector for human epidermal growth factor[J]. Journal of Zhengzhou University: Med Sci, 2004, 39(4): 603-605 |
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| Authors: | ZHAO Guoqiang ) ZHANG Lihua ) WANG Li ) BAO Yuzhou ) ) |
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| Affiliation: | ZHAO Guoqiang 1),ZHANG Lihua 2),WANG Li 2),BAO Yuzhou 2) 1)Department of Microbiology & Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 2)Laboratory of Molecular Biology,Henan Provincial Hospital,Henan Institute of Ophthalmology,Zhengzhou 450003 |
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| Abstract: | Aim: To construct eukaryotic expression vector of human epidermal growth factor. Methods: The cDNA sequence of human epidermal growth factor was amplified by RT PCR and then cloned into PGEM T Easy vector. After the sequence was analyzed, it was cut by restriction endonuclease and subcloned into eukaryotic expression vector pcDNA3.1. Results: The target fragment could be found when recombination had transformed into the competent cell E.coli JM109, then was cut by restriction endonuclease and identified by PCR. Conclusion: The eukaryotic expression vector of human epidermal growth factor has been successfully constructed and it could lay the foundation for future research. |
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| Keywords: | human epidermal growth factor clone gene expression |
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