高强度聚焦超声联合丝裂霉素治疗小鼠膀胱肿瘤的实验研究

目的　研究高强度聚焦超声 (HIFU)联合丝裂霉素对小鼠膀胱肿瘤的治疗作用和协同效应。　方法　制作小鼠膀胱肿瘤 (BTT739)皮下种植模型共 37只 ,随机分对照组、低剂量化疗组、高剂量化疗组、HIFU治疗组和HIFU联合化疗组。观察两周内种植瘤体积增长情况 ,计算肿瘤体积倍增时间 ,并作出生长曲线。摘除肿瘤瘤块进行病理学检查。　结果　HIFU联合化疗组对小鼠肿瘤的抑制作用优于单独化疗组和HIFU治疗组。病理学检查发现HIFU造成肿瘤组织大片凝固性坏死 ,肿瘤边缘及坏死区之间有少量活细胞残留 ,但凋亡细胞明显增多 ,增殖细胞核抗原 (PCNA)阳性率降低。　结论　HIFU和丝裂霉素联合治疗对小鼠肿瘤的抑制作用存在显著的协同效应 (P <0 0 1)。HIFU对小鼠肿瘤的抑制作用 ,除杀灭肿瘤细胞外 ,还发生肿瘤细胞凋亡现象。

An experimental study on high intensity focused ultrasound combined with mitomycin treatment of bladder tumor

Objective To study the therapeutic effect and synergistic nhibition effect of high intensity focused ultrasound in combination with mitomycin on T739 mice bladder tumor. Methods BTT739 tumor-bearing mice receiving HIFU and/or mitomycin in two weeks, were divided into control group, low dose chemotherapy group, high dose chemotherapy group, HIFU group and HIFU combined chemotherapy group. The growth of mice tumor volume was observed in two weeks, by which we counted tumor volume doubling time and performed the growth curve. All specimens were analysed histologically. Results HIFU combined mitomycin has significant synergistic inhibition effect. Tumor tissue damage such as huge coagulation necrosis was observed using light microscopy. However, there were still some remaining alive cells. The apoptosis of tumor cell in HIFU group and HIFU combined chemotherapy group obviously increased in comparison with other groups. Conclusions HIFU group, HIFU combined chemotherapy group can distinctively inhibit tumor growth; HIFU combined with mitomycin has notable synergistic inhibitory effect. HIFU may induce the apoptosis of tumor cell.