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| 联合应用磁性纳米Fe3O4颗粒和5溴汉防己甲素对柔红霉素诱导的K562／A02细胞凋亡效应的影响 | |
| 引用本文： | 沈明芳,陈宝安,程坚,高峰,许文林,丁家华,高冲,孙新臣,李国宏,陈文姬,刘莉洁,李小毛,王雪梅.联合应用磁性纳米Fe3O4颗粒和5溴汉防己甲素对柔红霉素诱导的K562／A02细胞凋亡效应的影响[J].中国实验血液学杂志,2009,17(1):54-59. |
| 作者姓名： | 沈明芳 陈宝安 程坚 高峰 许文林 丁家华 高冲 孙新臣 李国宏 陈文姬 刘莉洁 李小毛 王雪梅 |
| 作者单位： | 1. 东南大学中大医院血液科,江苏南京,210009 2. 江苏大学人民医院血液科,江苏镇江,212002 3. 东南大学中大医院肿瘤科,江苏南京,210009 4. 东南大学生理教研室,江苏南京,210009 5. 萨尔大学医学部,德国萨尔布吕肯D-66041 6. 东南大学生物电子学国家重点实验室,江苏南京,210096 |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，Higll School Doctor Subjec Specia-purpose Scientific Research Foundation |
| 摘 要： | 本研究探讨磁性纳米Fe3O4颗粒MNP（Fe3O4）]和5溴汉防己甲素（5-BrTet）联合治疗慢性髓系白血病的潜在可能性。采用流式细胞术、Wright染色和光学显微镜检测细胞凋亡情况；采用Western blot法检测细胞BCL-2和BAX表达水平。结果表明：MNP（Fe3O4）或5-BrTet与柔红霉素（DNR）联用对K562／A02细胞有细胞毒作用，而联合应用MNP（Fe3O4）和5-BrTet可协同促进DNR诱导K562／A02细胞凋亡，且细胞出现典型的凋亡形态改变，同时可见BCL-2表达下调，BAX表达上调。结论：MNP（Fe3O44）或5-Br/Tet联合DNR均能有效促进K562／A02细胞凋亡。两者联合应用作用增强，其机制可能与BCL-2表达下调及BAX表达上调有关。 |
| 关 键 词： | K562／A02白血病细胞 多药耐药 磁性纳米Fe3O4颗粒 5溴汉防己甲素 柔红霉素 细胞凋亡 BCL-2 BAX |
Effects of Magnetic Nanoparticle of Fe3O4 and 5-Bromotetrandrin on Apoptosis of K562/A02 Leukemic Cells Induced by Daunorubicin |
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| SHEN Ming-Fang,CHEN Bao-An,CHENG Jian,GAO Feng,XU Wen-Lin,DlNG Jia-Hua,GAO Chong,SUN Xin-Chen,LI Guo-Hong,CHEN Wen-Ji,LIU Li-jie,LI Xiao-Mao,WANG Xue-Mei.Effects of Magnetic Nanoparticle of Fe3O4 and 5-Bromotetrandrin on Apoptosis of K562/A02 Leukemic Cells Induced by Daunorubicin[J].Journal of Experimental Hematology,2009,17(1):54-59. | |
| Authors: | SHEN Ming-Fang CHEN Bao-An CHENG Jian GAO Feng XU Wen-Lin DlNG Jia-Hua GAO Chong SUN Xin-Chen LI Guo-Hong CHEN Wen-Ji LIU Li-jie LI Xiao-Mao WANG Xue-Mei |
| Institution: | (Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical School, Nanjing, 210009, Jiangsu Province, China; 1.Department of Hematology, People Hospital, Jiangsu University, Zhenjiang 212002, Jiangsu Province, China; 2.Department of Oncology, Zhongda Hospital, Southeast University Clinical Medical School, Nanjing 210009, Jiangsu Province, China ; 3.Department of Physiology, Southeast University, Nanjing 210009, Jiangsu Province, China ; 4.Department of Physics, University of Saarland, D-66041 Saarbruechen, Germany; 5.National Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, Jiangsu Province, China) |
| Abstract: | The aim of this study was to investigate the potential benefit of combination therapy with magnetic nanop article of Fe3O4 and 5-Bromotetrandrine(5-BrTet)on chronic leukemia.The apoptosis was detected by flow cytometry (FCM),Wright staining and light microscope:the expressions of BAX and BCL-2 were measured bv western blot.The results showed that combination of daunorubicin(DNR)witll either MNP(Fe3O4)or 5-BrTet exeaed a potent cytotoxic effect on K562/A02 cells,while MNP(Fe3O4)and 5-BrTet co-treatment could synergistically enhance DNR-induced ap optosis.After treated with this regimen,the typical apoptotic morphological features were found in K562/A02 cells:the expression level of BCL-2 decreased and BAX increased markedly.It is concluded that MNP(Fe3O4)or 5-BrTet with DNR can induce apoptosis in K562/A02 cells.and they show distinct synergism when used together.The down-regula tion of BCL-2 and the up-regulation of BAX may play important roles. |
| Keywords: | BCL-2 BAX K562/A02 leukemic cell muitidrug resistance magnetic nanoparticle of Fe3O4 5-Bromotetrandrine daunorubicin apoptosis BCL-2 BAX |
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