HPLC法测定太子参黄芪口服液中太子参环肽B的含量 |
| |
引用本文: | 庞永琴,瞿京红. HPLC法测定太子参黄芪口服液中太子参环肽B的含量[J]. 国际中医中药杂志, 2016, 0(9): 841-843. DOI: 10.3760/cma.j.issn.1673-4246.2016.09.020 |
| |
作者姓名: | 庞永琴 瞿京红 |
| |
作者单位: | 湖北省湖北医药学院附属东风医院儿童诊疗中心,十堰,442008 |
| |
基金项目: | 湖北省2011协同创新中心基金(4) |
| |
摘 要: | 目的:建立太子参黄芪口服液中太子参环肽B含量测定的HPLC方法。方法采用Phenomenex Luna-C18柱(4.6 mm×250 mm,5μm),以乙腈-0.1%醋酸溶液为流动相,梯度洗脱,流速1.0 ml/min,柱温30℃,检测波长203 nm。结果太子参环肽B在0.016~0.320 mg/ml浓度范围内有良好线性关系(r=0.9998),平均加样回收率为98.92%,RSD为0.80%。检测5批太子参黄芪口服液中太子参环肽B的平均含量为1.63 mg/ml。结论本方法灵敏度高、准确、重复性好、专属性强,可用于太子参黄芪口服液的质量控制。
|
关 键 词: | 太子参环肽B 太子参黄芪口服液 质量控制 高效液相色谱 |
Determination of heterophyllin B inTaizishen-Huangqi oral solution by HPLC |
| |
Abstract: | Objective To study a method for the determination of heterophyllin B inTaizishen- Huangqi oral solution.Methods The HPLC system consisted of Phenomenex Luna-C18 (4.6 mm × 250 mm, 5μm) column, the mobile phase consisted of acetonitrile and 0.1% acetic acid, the gradient elution flow rate was 1.0 ml/min, the column temperature was 30℃, and the UV detector was set at 203 nm.Results The linear response range was 0.016-0.320 mg/ml (r=0.999 8). The average recovery of heterophyllin B was 98.92%, and RSD was 0.80%. The average determination of Heterophyllin B within 5 batches of preparation was 1.63 mg/ml. Conclusions The method showed its high sensitivity, accurate, repeatable and high secificity. It could be used as a method to control the standard ofTaizishen-Huangqi oral solution. |
| |
Keywords: | Heterophyllin B Taizishen-Huangqi oral solution Quality control High performance liquid chromatography |
本文献已被 万方数据 等数据库收录! |