中药接骨颗粒对家兔肾虚骨折愈合的组织形态学影响

为研究中药接骨颗粒对家兔肾虚骨折愈合的组织形态学影响。将30只家兔随机分成对照组、肾虚组、中药组。各组均造成桡骨中段7mm缺损。肾虚组、中药组口服泼尼松造肾虚动物模型,中药组加服中药接骨颗粒。6周后,取完整双侧尺桡骨,剔除附着的软组织,拍X线片,评价骨折愈合情况;将骨标本处理、切片、染色后,进行组织形态学观察。结果显示,经X线片评价优良率对照组为80%、肾虚组为63%、中药组为75%。组织形态学观察,对照组骨痂已部分骨化,中心区有软骨性组织和纤维性组织,ALP检查呈棕黑色颗粒;肾虚组骨痂以软骨性组织为主,较多为未成熟胶原,ALP染色仅少量面积,且染色浅;中药组有不同程度骨痂生长,骨痂内成熟胶原成分较多,ALP染色较浅,但面积广泛。表明实验用中药接骨颗粒有纠正肾虚的良好作用。

HISTOMORPHOLOGICAL EFFECT OF "JIEGU KELI" ON FRACTURE HEALING IN RABBIT WITH KIDNEY DEFICIENCY

In order to study the histomorphological effect of "JIEGU KELI" (JGK,TCD granulae for fracture healing) on fracture healing,30 rabbits were made into animal models of 7-mm middle-radius defect and randomly divided into three groups: the control,the kidney-deficiency and the TCD-treated;two of the latter were given Prednisone as to cause the kidney-deficiency models,the TCD-treated were given JGK besides and the control was given only routine forage;6 weeks later,the whole bilateral ulnae ad radii were excised,the fracture healings of the specimens were evaluated by the X-rays and the histomorphological observations were made on the treated,sliced and stained specimens.The results showed(1) the fracture-healing good-excellent rates of the control,the kidney-deficiency and the TCD-treated: 80 %,63 % and 75 %,respectively;(2) the histomorphological findings: in the control-partly ossified callus with cartilageous and fibrous tissues in the center and brown-black granulae shown by ALP staining;in the kidney-deficiency-callus composed of cartilage tissues and immature collages and less ALP-understained areas;in the TCD-treated-varied-degree grown callus composed mostly of mature collage and ALP-understained but extensive areas,suggesting that JGK has a good function to correct the kidney deficiency.