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| 苯酚-硫酸法测定杂多糖含量的研究 | |
| 引用本文： | 杨勇杰,姜瑞芝,陈英红,张萍,高其品. 苯酚-硫酸法测定杂多糖含量的研究[J]. 中成药, 2005, 27(6): 706-708 |
| 作者姓名： | 杨勇杰 姜瑞芝 陈英红 张萍 高其品 |
| 作者单位： | 1. 吉林大学生命科学院,吉林,长春,130023;吉林省中医中药研究院,吉林,长春,130021 2. 吉林省中医中药研究院,吉林,长春,130021 3. 中国科学院长春应用化学研究所,吉林,长春,130022 4. 吉林省药品检验所,吉林,长春,130062 |
| 摘 要： | 目的:确立一种能够准确测定杂多糖中总糖含量的实验方法.方法:采用酸水解多糖,GC法分析各组成糖比例,配制相应的标准溶液,用苯酚-硫酸法测定多糖含量.结果:实验结果良好,标准糖混合溶液在8.56～38.52 mg·L-1浓度范围内呈线性关系.结论:此法测定结果真实、准确,适用于测定杂多糖的总糖含量. |
| 关 键 词： | 苯酚-硫酸法 校正因子 气相色谱 |
| 文章编号： | 1001-1528(2005)06-0706-03 |
Determination of sugars in heteropolysaccharide by phenol-sulfuric acid method |
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| YANG Yong-jie,JIANG Rui-zhi,CHEN Ying-hong,ZHANG Ping,GAO Qi-pin. Determination of sugars in heteropolysaccharide by phenol-sulfuric acid method[J]. Chinese Traditional Patent Medicine, 2005, 27(6): 706-708 | |
| Authors: | YANG Yong-jie JIANG Rui-zhi CHEN Ying-hong ZHANG Ping GAO Qi-pin |
| Abstract: | AIM: To develop a new method based on phenol-sulfuric reaction for the determination of hetero-polysaccharide. METHODS: The proportion of sugars in the hetero-polysaccharide was determined by GC. According to standard solution obtained, Heteropolysaccharide can be determined through the standard curve. ( RESULTS:)The linear relationship of standard solution of mixed sugars was at the range of 8.56-38.52 mg/L~(-1). CONCLUSION: GC and phenol-sulfuric acid method can be used to determine the heteropolysaccharide accurately. |
| Keywords: | GC phenol-sulfuric acid recovery factor |
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