UPLC法测定不同产地五指毛桃中芹菜素等6个黄酮含量

目的:建立超高效液相色谱法测定五指毛桃中6个黄酮成分的含量,比较不同产地的五指毛桃中芹菜素等6个黄酮类成分的含量差别并作聚类分析。方法:色谱柱:ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm);流动相A为甲醇-乙腈(1∶1)溶液,B为0.1%磷酸水溶液,梯度洗脱,检测波长为365 nm,柱温为30℃,流速为0.5 mL·min^-1,进样体积为10μL。结果:6个黄酮化合物均能实现完全分离,且呈现良好线性关系,回收率在98.53%~100.15%;不同产地五指毛桃中的6个黄酮类成分含量具有差异,聚类分析结果表明,五指毛桃具有明显的产地聚类特性。结论:该方法操作简便,准确可靠,重复性好,专属性强,并且分析时间短,适合五指毛桃的含量测定,且因不同产地质量差异明显,可以通过产地优选来保证五指毛桃来源的质量稳定性。

Establishment of an Ultra Performance Liquid Chromatography (HPLC) Method to Determine the Contents of 6 Flavonoids in Ficus hirta from Different Habitats

Objective:To establish an ultra performance liquid chromatography(UPLC)method to determine 6 flavonoids in Ficus hirta,and to cluster analyze their content differences from different habitats.Methods:Flavonoids were determined by UPLC using an ACQUITY UPLC BEH C18 column(2.1 mm×50 mm,1.7μm)with mobile phase A(methanol∶acetonitrile,1∶1)and B(0.1%phosphoric acid aqueous solution)eluted gradiently at 30℃.The detection wavelength was 365 nm.The cluster analysis of samples from different origins was carried out employing the IBM SPSS 20.0 software.Results:All the 6 flavonoids were completely separated and showed good linear relationship.The recovery rates ranged from 98.53%to 100.15%.Clustering analysis based on the contents of the six flavonoids showed that Ficus hirta from different habitats had obvious clustering characteristics.Conclusion:The established UPLC method is simple,accurate,repeatable,specific and fast,which is suitable for the quality control and evaluation of Ficus hirta.The quality difference of samples form different habitats is obvious,so it is possible to ensure the quality stability and drug safety of Ficus hirta by optimizing the producing area.