太子参粗多糖对大鼠急性心肌梗死诱发心肺损伤的保护作用

目的:研究太子参粗多糖对急性心肌梗死后诱发实验性大鼠心肺损伤的保护作用。方法:采用大鼠左冠状动脉结扎复制急性心肌梗死模型,持续4周形成心肺损伤。血流动力学指标分析心功能;重量计算法分析心脏指数、左心室指数和肺指数;病理组织学分析左心室和肺病理改变;图像分析系统分析心肌梗死面积。结果:与假手术组比较,急性心肌梗死4周后,模型组大鼠心功能紊乱,心指数和肺指数增加,病理组织学分析证实左心室组织心肌细胞减少、成纤维细胞增生、炎细胞浸润,肺组织充血、水肿、炎细胞浸润,提示急性心肌梗死诱发心肺损伤病变模型成功。太子参粗多糖连续灌胃4周后,显著改善上述指标,与模型组比较,差异显著(P0.05,P0.01)。结论:太子参粗多糖对急性心肌梗死诱发实验性大鼠心肺损伤具有一定的保护作用,为太子参益气健脾、生津润肺的功效物质基础提供现代科学认识。

Protective effects of crude polysaccharides from Radix Pseudostellariae on rat experimental cardiopulmonary injury induced by acute myocardial infarction in vivo

Objective:To study the protective effects of crude polysaccharides from Radix Pseudostellariae(RP) on rat experimental cardiopulmonary injury induced by acute myocardial infarction in vivo.Methods:The cardiopulmonary injury were reproduced after 4 weeks by ligatured the left coronary artery in rats.The cardiac function was assayed by hemodynamics,heart index,ventriculus sinister index and lung index were detected by weight,pathologic change was checked out by histophathology,and cardiac infarction size was assayed by image analysis system.Resutls:After 4 weeks of operation,cardiac function was disturbed according to the results of hemodynamics.Cardiac and lung index,and cardiac infarction size was significantly increased in model group.Results of histopathology confirmed that cardiac myocyte decreased,cardiac fibrosis increased,inflammatory cell infiltration,and pulmonary congestion and edema,which indicate the model was succeed.After continued administrating crude polysaccharides from Radix Pseudostellariae 4 weeks it can remarkable ameliorate cardiac function,decrease the cardiac and lung index,and attenuate the heart failure pathological change.Conclusion:Crude polysaccharides from Radix Pseudostellariae can alleviate rat cardiopulmonary injury induced by acute myocardial infarction,the present research provide the pharmacological base of bioactivity ingredients for replenishing qi to invigorate the spleen and promoting the production of body fluid and nourishing the lung.