改良线栓法制作大鼠局灶性脑缺血模型

目的：探索一种简便可重复的脑缺血动物模型建立方法。方法：选用250～300g　SD成年大鼠20只，采用改良的Longa线栓法制作大鼠局灶性脑缺血模型，并评价缺血效果。结杲：术后大鼠均出现了偏瘫等神经功能障碍，镜下观察可见大鼠大脑左侧额顶皮质以及基底节区大范围的梗死区，呈现白色。结论：神经功能损害以及1％　TTC大鼠脑切片染色显示改良线栓法建立局灶性脑缺血模型效果较好。

Establishment of focal ischemic model with reforming Longa method in rats

Objective :To explore a reliable method of focal cerebral ischemia in rats.Methods :Focal cere-bral ischemia model was made by reforming Longa methodin Sprague-Dawley rats and neurological deficitof all rats were graded after suture occluded.Internal carotid artery and common carotid artery were tiedwith suture which was retracted by forceps in order to reduce the bleedingin process.External artery wasligated and cut 2mmlong before the beginning of bifurcation of common carotid artery.The obstruction ofthe middle cerebral artery was performed byinserting 0.2mm monofilament nylon suture in the left inter-nal caroted artery via external carotid artery until it reach and occlude the ostiumof theleft middle cerebralartery.Result :All rats were made semi paralyzed after procedure.Conclusion:It is a reliable way to madefocal ischemic model by reforming Longa method that focal ischemic lesion well set up with neurologicaldeficit in rats.