外周血单核细胞SOCS-1表达与MODS患者预后关系的研究

目的了解外周血单核细胞SOCS-1表达与多器官功能障碍综合征（MODS）患者预后的关系及临床意义。方法收集24例MODS患者，并采集其外周静脉血，采用淋巴细胞分离液密度梯度离心法分离外周血单核细胞（PBMCs），分别以RT-PCR法及Western-blot法检测PBMCs中SOCS-1的基因及蛋白表达，分析其与预后及MODS评分的关系。结果MODS组中死亡患者PBMCs中的SOCS-1mRNA表达量（0.4938±0.0273）显著低于存活患者（0.5475±0.0289）（P〈0.05），SOCS-1蛋白表达量（0.7924±0.0284）显著低于存活患者（0.8406±0.0407）（P〈0.05）。MODS患者的PBMCs中的SOCS-1mRNA表达量与MODS评分呈显著的负相关关系（r=-0.723,P〈0.01），SOCS-1蛋白表达量与MODS评分呈显著的负相关关系（r=-0.534，P〈0.01）。结论在MODS中，SOCS-1的表达可能起到保护组织避免损伤的作用,SOCS-1表达的减少可能提示患者的预后不良

Prognostic Value of SOCS-1 Expression by Peripheral Blood Mononuclear Cells in Patients with Multiple Organ Dysfunction Syndrome

Objective To evaluate the prognostic value of SOCS-1 in patients with multiple organ dysfunction syndrome （MODS）. Methods Peripheral blood of 24 patients with MODS was collected and the peripheral blood mononuclear cells （PBMCs） were isolated by Ficoll-Hypaque centrifugation method. The mRNA of SOCS-1 was determined by RT-PCR. And the protein content of SOCS-1 was determined by Western blot. Results The mRNA （0.4938±0.0273） and protein （0.7924±0.0284） level of SOSC-1 in the PBMCs of patients died of MODS was significant lower than the PBMCs obtained from the surviving patients （both P〈0.05）. There was a negative correlation between the SOCS-1 mRNA expression and the MODS score （r=-0.723, P〈0.01 ）. There was also a significant negative correlation between the SOCS-1 content and the MODS score （r=-0.534,P〈0.01）. Conclusion SOCS-1 may protect the tissue from further damage. A low expression level of SOCS-1 may serve as a prognostic indicator in patients with MODS.